Multi-walled carbon nanotubes inhibit regenerative axon growth of dorsal root ganglia neurons of mice.
Multi-walled carbon nanotubes inhibit regenerative axon growth of dorsal root ganglia neurons of mice.
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DOI：
10.1016/j.neulet.2011.11.056
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发表时间：
2012-01-17
影响因子：
2.5
通讯作者：
Murashov AK
中科院分区：
文献类型：
--
作者：
Wu D;Pak ES;Wingard CJ;Murashov AK
最近的观察表明，纳米材料可能对人体组织有毒。虽然已知纳米级颗粒物的能力会导致呼吸系统的一系列问题，但最近的观察表明，神经系统也可能很脆弱。在目前的论文中，我们询问了原代神经元细胞培养物暴露于纳米颗粒是否可能损害再生轴突生长。再生反应是通过在收集背根神经节（DRG）前5天对坐骨神经进行条件性损伤来触发的。将DRG神经元以低密度铺板，并与多壁碳纳米管（MWCNT）（0.1 - 10 μg/ml，在10%表面活性剂的盐水溶液中）一起孵育过夜。实验表明，DRG培养物暴露于MWCNT显著损害再生轴突发生，而不伴随细胞死亡。这些结果表明，MWNCTs可能对神经再生产生不利影响，并可能潜在地引发轴突病理学。
Recent observations have demonstrated that nanomaterials may be toxic to human tissue. While the ability of nano-scaled particulate matter is known to cause a range of problems in respiratory system, recent observations suggest that the nervous system may be vulnerable as well. In the current paper we asked whether exposure of primary neuronal cell cultures to nanoparticles might compromise regenerative axon growth. Regenerative response was triggered by performing a conditioning lesion of sciatic nerve five days prior to collection of dorsal root ganglia (DRG). DRG neurons were plated at a low density and incubated with multi-walled carbon nanotubes (MWCNT) (0.1 – 10 μg/ml in 10% of surfactant in saline) overnight. The experiments showed that exposure of DRG cultures to MWCNT significantly impaired regenerative axonogenesis without concomitant cell death. These results indicate that MWNCTs may have detrimental effect on nerve regeneration and may potentially trigger axonal pathology.
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