Polymorphic chromosomal inversions in Anopheles moucheti, a major malaria vector in Central Africa.

Polymorphic chromosomal inversions in Anopheles moucheti, a major malaria vector in Central Africa.
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DOI:
10.1111/mve.12037
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发表时间:
2014-09
影响因子:
1.9
通讯作者:
Sharakhov IV
Sharakhov IV
中科院分区:
农林科学2区
文献类型:
--
作者:
Sharakhova MV;Antonio-Nkondjio C;Xia A;Ndo C;Awono-Ambene P;Simard F;Sharakhov IV

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莫氏按蚊(Anopheles moucheti Evans)(双翅目,蚊科)是中非森林地区的主要疟疾媒介。然而,很少有遗传工具可用于该物种。本研究是首次尝试在AN染色体特征。在喀麦隆采集的雌猴。卵巢滋养细胞含有多线染色体,适用于标准的细胞遗传学应用。三个多态性染色体倒位的存在,在An。穆切蒂透露。其中两个倒位位于2R染色体臂上。moucheti和An. gambiaeGiles的荧光原位杂交建立。冈比亚基因序列。探针在安氏按蚊染色体上的定位。Moucheti检测到这两个物种之间大量的基因顺序重组。多线染色体和多态倒位的存在。moucheti为这一被忽视的疟疾病媒的种群遗传学、分类学和生态学研究提供了一种新工具。
Anopheles moucheti Evans (Diptera, Culicidae) is a major vector of malaria in forested areas of Central Africa. However, few genetic tools are available for this species. The present study represents the first attempt to characterize chromosomes in An. moucheti females collected in Cameroon. Ovarian nurse cells contained polytene chromosomes, which were suitable for standard cytogenetic applications. The presence of three polymorphic chromosomal inversions in An. moucheti was revealed. Two of these inversions were located on 2R chromosomal arm. The homology between 2R chromosomal arms of An. moucheti and An. gambiae Giles was established by fluorescent in situ hybridization of six An. gambiae genic sequences. Mapping of the probes on chromosomes of An. moucheti detected substantial gene order reshuffling between the two species. The presence of polytene chromosomes and polymorphic inversions in An. moucheti provides a new tool for population genetic, taxonomic, and ecological studies of this neglected malaria vector.
DOI: 10.1186/1475-2875-7-120
发表时间: 2008-07-04
期刊: MALARIA JOURNAL
影响因子: 3
作者:
Antonio-Nkondjio, Christophe;Ndo, Cyrille;Kengne, Pierre;Mukwaya, Louis;Awono-Ambene, Parfait;Fontenille, Didier;Simard, Frederic
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发表时间: 2002-11-15
期刊: SCIENCE
影响因子: 56.9
作者:
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