Regeneration and transdetermination: the role of wingless and its regulation.
Regeneration and transdetermination: the role of wingless and its regulation.
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DOI：
10.1016/j.ydbio.2010.08.034
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发表时间：
2010-11-15
影响因子：
2.7
通讯作者：
Schubiger G
中科院分区：
文献类型：
--
作者：
Schubiger M;Sustar A;Schubiger G
果蝇的想象盘具有非凡的再生能力。碎裂伤愈合后，异位wg被诱导并形成芽基。在一些但不是所有的碎片中，芽基将取代缺失的结构，少数细胞可以变得更具可塑性并转化为其他圆盘的结构。通过第一腿椎间盘的一系列系统性切割显示，切割必须横切背侧近端椎间盘区域，并且碎片还必须包括WG感受态细胞。当提供有外源Wg时，既不能进行转决定又不能再生缺失结构的片段将进行转决定和再生缺失结构，这证明了Wg在再生过程中的必要性。在完整的腿盘普遍表达低水平的Wg也导致芽基的形成，再生和transdetermination。外源性wg诱导后两天，内源性基因被激活，导致腿盘背侧Wg水平升高。我们鉴定了一个调节异位wg表达的wg增强子。该增强子的缺失增加了转决定，但降低了异位Wg的量。我们推测，这减少了对DPP背侧的抑制，从而创造了一个允许的条件下，异位Wg和DPP的平衡有利于transdetermination。
Imaginal discs of Drosophila have the remarkable ability to regenerate. After fragmentation wound healing occurs, ectopic wg is induced and a blastema is formed. In some, but not all fragments, the blastema will replace missing structures and a few cells can become more plastic and transdetermine to structures of other discs. A series of systematic cuts through the first leg disc revealed that a cut must transect the dorsal-proximal disc area and that the fragment must also include wg-competent cells. Fragments that fail to both transdetermine and regenerate missing structures will do both when provided with exogenous Wg, demonstrating the necessity of Wg in regenerative processes. In intact leg discs ubiquitously expressed low levels of Wg also leads to blastema formation, regeneration and transdetermination. Two days after exogenous wg induction the endogenous gene is activated, leading to elevated levels of Wg in the dorsal aspect of the leg disc. We identified a wg enhancer that regulates ectopic wg expression. Deletion of this enhancer increases transdetermination, but lowers the amount of ectopic Wg. We speculate that this lessens repression of dpp dorsally, and thus creates a permissive condition under which the balance of ectopic Wg and Dpp is favorable for transdetermination.
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影响因子：
64.8
作者：
HOOPER, JE
通讯作者：
HOOPER, JE
影响因子：
2.7
作者：
BRYANT, PJ
通讯作者：
BRYANT, PJ
影响因子：
2.7
作者：
Bosch, M;Serras, F;Baguñà, J
通讯作者：
Baguñà, J
DOI：
10.1007/bf00848779
发表时间：
1977-01-01
期刊：
WILHELM ROUXS ARCHIVES OF DEVELOPMENTAL BIOLOGY
影响因子：
--
作者：
HAYNIE, JL;BRYANT, PJ
通讯作者：
BRYANT, PJ
影响因子：
56.9
作者：
Gurley, Kyle A.;Rink, Jochen C.;Alvarado, Alejandro Sanchez
通讯作者：
Alvarado, Alejandro Sanchez