DNA origami with double-stranded DNA as a unified scaffold.
DNA origami with double-stranded DNA as a unified scaffold.
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DOI：
10.1021/nn302896c
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发表时间：
2012-09-25
期刊：
影响因子：
17.1
通讯作者：
Yan, Hao
中科院分区：
文献类型：
--
作者：
Yang, Yang;Han, Dongran;Nangreave, Jeanette;Liu, Yan;Yan, Hao
支架DNA折纸是一种广泛使用的技术，用于自组装包含大量可寻址特征的精确结构的纳米级物体。典型的支架是长的单链DNA（ssDNA），其通过与支架的几个不同结构域互补的许多短ssDNA斯台普斯的作用折叠成不同的形状。然而，长单链DNA的来源是稀缺的，限制了可以组装的结构的大小和复杂性。在这里，我们证明了dsDNA支架可以直接用于制造整合的DNA折纸结构，将两个组成ssDNA分子。在支架折叠路径的设计中采用了两个基本原则-折叠路径不对称性和两个ssDNA支架链的周期性收敛。折叠路径中的不对称性使斯台普斯之间的不需要的互补性最小化，并且在每条ssDNA支架链的折叠路径之间并入偏移减少了链的互补部分彼此紧密接近的次数，这两者都降低了dsDNA支架恢复的可能性。同时，两个ssDNA支架链的折叠路径被设计成周期性地会聚，以促进单个统一结构的组装，而不是两个单独的结构。我们的研究结果表明，这种基本策略可以用来可靠地组装集成的DNA纳米结构从dsDNA支架。
Scaffolded DNA origami is a widely used technology for self-assembling precisely structured nanoscale objects that contain a large number of addressable features. Typical scaffolds are long, single strands of DNA (ssDNA) that are folded into distinct shapes through the action of many, short ssDNA staples that are complementary to several different domains of the scaffold. However, sources of long single stranded DNA are scarce, limiting the size and complexity of structures that can be assembled. Here we demonstrated that dsDNA scaffolds can be directly used to fabricate integrated DNA origami structures that incorporate both of the constituent ssDNA molecules. Two basic principles were employed in the design of scaffold folding paths – folding path asymmetry and periodic convergence of the two ssDNA scaffold strands. Asymmetry in the folding path minimizes unwanted complementarity between staples, and incorporating an offset between the folding paths of each ssDNA scaffold strand reduces the number of times that complementary portions of the strands are brought into close proximity with one another, both of which decrease the likelihood of dsDNA scaffold recovery. Meanwhile, the folding paths of the two ssDNA scaffold strands were designed to periodically converge to promote the assembly of a single, unified structure rather than two individual ones. Our results reveal that this basic strategy can be used to reliably assemble integrated DNA nanostructures from dsDNA scaffolds.
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影响因子：
15
作者：
Ke, Yonggang;Douglas, Shawn M.;Liu, Minghui;Sharma, Jaswinder;Cheng, Anchi;Leung, Albert;Liu, Yan;Shih, William M.;Yan, Hao
通讯作者：
Yan, Hao
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作者：
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影响因子：
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作者：
Ke, Yonggang;Sharma, Jaswinder;Yan, Hao
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影响因子：
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作者：
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通讯作者：
Shih, William M.
影响因子：
64.8
作者：
Rothemund, PWK
通讯作者：
Rothemund, PWK