Temporally-regulated quick activation and inactivation of Ras is important for olfactory behaviour.
Temporally-regulated quick activation and inactivation of Ras is important for olfactory behaviour.
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DOI：
10.1038/srep00500
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发表时间：
2012
影响因子：
4.6
通讯作者：
Ishihara, Takeshi
中科院分区：
文献类型：
--
作者：
Uozumi, Takayuki;Hirotsu, Takaaki;Yoshida, Kazushi;Yamada, Ryuji;Suzuki, Akiya;Taniguchi, Gun;Iino, Yuichi;Ishihara, Takeshi
对环境刺激的反应由各种信号蛋白的激活和失活介导。然而，这些事件在活体动物中的时间动态还没有得到很好的理解。在这里，我们显示了实时成像的活性的MAP激酶途径，Ras，在活的秀丽隐杆线虫和Ras是短暂激活的嗅觉神经元在几秒钟内响应于增加的气味浓度。Ras的这种快速激活依赖于嗅觉信号通路和Ras鸟苷酸释放蛋白（Ras GRP）。负反馈循环然后迅速导致Ras失活，尽管气味剂持续存在。Ras突变体的表型表明Ras的这种快速激活和失活对于调节中间神经元活动和嗅觉行为是重要的。我们的研究结果揭示了新的动力学和嗅觉系统中Ras的瞬时激活的生物学意义。
Responses to environmental stimuli are mediated by the activation and inactivation of various signalling proteins. However, the temporal dynamics of these events in living animals are not well understood. Here we show real-time imaging of the activity of the key regulator of the MAP kinase pathway, Ras, in living Caenorhabditis elegans and that Ras is transiently activated within a few seconds in olfactory neurons in response to increase in the concentration of odorants. This fast activation of Ras is dependent on the olfactory signalling pathway and Ras guanyl nucleotide-releasing protein (RasGRP). A negative feedback loop then quickly leads to Ras inactivation despite the continued presence of the odorant. Phenotypes of Ras mutants suggest this rapid activation and inactivation of Ras is important for regulation of interneuron activities and olfactory behaviours. Our results reveal novel kinetics and biological implication of transient activation of Ras in olfactory systems.
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影响因子：
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通讯作者：
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