Synthesis, isolation, and characterization of conjugates of ovalbumin with monomethoxypolyethylene glycol using cyanuric chloride as the coupling agent.
Synthesis, isolation, and characterization of conjugates of ovalbumin with monomethoxypolyethylene glycol using cyanuric chloride as the coupling agent.
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使用氰尿酰氯作为偶联剂合成、分离和表征卵清蛋白与单甲氧基聚乙二醇的缀合物。
DOI：
10.1016/0003-2697(87)90208-9
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发表时间：
1987
影响因子：
2.9
通讯作者：
Sehon,AH
中科院分区：
文献类型：
--
作者：
Jackson,CJ;Charlton,JL;Kuzminski,K;Lang,GM;Sehon,AH
研究了使用氰尿酰氯作为偶联剂制备预选平均缀合度 n 的卵清蛋白 (OA) 和单甲氧基聚乙二醇 (mPEG) 缀合物的实验条件，重点是产物的纯化和表征。这些缀合物充当抗原蛋白的耐受性mPEG衍生物的原型，其能够抑制哺乳动物对相应未修饰抗原的免疫反应。在该实验室的其他研究中，发现 OA(mPEG)n 缀合物的耐受原性是 n 的函数。结果表明，产生 OA(mPEG)n 缀合物的反应的重现性主要取决于 mPEG-氰尿酰氯中间体的反应性，为了获得最佳结果，必须在完全无水条件下合成该中间体。通过使用离子交换色谱法优化 OA(mPEG)n 缀合物的分离，从而实现从缀合物中快速去除大量未偶联的中间体；分馏条件受共轭程度的影响。这种纯化方法优于透析、超滤和凝胶过滤。此外，通过应用分析性疏水相互作用HPLC，可以区分不同缀合程度的缀合物，并确定任何制剂中不存在任何可检测到的游离OA。缀合物中 mPEG 的量直接通过 NMR 测定。
The experimental conditions for the preparation of conjugates of ovalbumin (OA) and monomethoxypolyethylene glycol (mPEG) of a preselected average degree of conjugation, n, using cyanuric chloride as the coupling agent, have been investigated with emphasis on purification and characterization of the products. These conjugates served as prototypes of tolerogenic mPEG derivatives of antigenic proteins which were capable of suppressing in mammals the immunological response to the corresponding unmodified antigens. In other studies in this laboratory, the tolerogenicity of OA(mPEG)nconjugates was found to be a function of n. The reproducibility of the reaction leading to the production of OA(mPEG)nconjugates was shown to depend primarily on the reactivity of the mPEG-cyanuric chloride intermediate, which—for best results—had to be synthesized under completely anhydrous conditions. Isolation of the OA(mPEG)nconjugates was optimized by the use of ion-exchange chromatography whereby rapid removal of large amounts of uncoupled intermediate from the conjugate was achieved; the conditions of fractionation were affected by the degree of conjugation. This method of purification was superior to dialysis, ultrafiltration, and gel filtration. Furthermore, by the application of analytical hydrophobic interaction HPLC it was possible to differentiate among conjugates of different degrees of conjugation and to establish the absence of any detectable free OA in any of the preparations. The quantity of mPEG in the conjugates was determined directly by NMR.
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利用三氯均三嗪制备生物特异性吸附剂和固定化酶的新方法。
影响因子：
2.9
作者：
T. Finlay;V. Troll;M. Levy;A. Johnson;L. T. Hodgins
通讯作者：
L. T. Hodgins
小鼠对蜜蜂毒液和单甲氧基聚乙二醇修饰的蜜蜂毒液的抗体反应。
DOI：
10.1159/000233395
发表时间：
1983
期刊：
International archives of allergy and applied immunology
影响因子：
--
作者：
S. Ahlstedt;B. Björkstén;E. Åkerblom
通讯作者：
E. Åkerblom
豚草抗原 E 与各种烷氧基聚乙二醇缀合物的免疫学特性。
DOI：
10.1159/000232852
发表时间：
1981
期刊：
International archives of allergy and applied immunology
影响因子：
--
作者：
T. P. King;C. Weiner
通讯作者：
C. Weiner
影响因子：
64.8
作者：
W. Y. Lee;A. Sehon
通讯作者：
A. Sehon
DOI：
10.1084/jem.149.2.424
发表时间：
1979-02-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
King TP;Kochoumian L;Chiorazzi N
通讯作者：
Chiorazzi N