A novel colorimetric biosensor for detecting SARS-CoV-2 by utilizing the interaction between nucleocapsid antibody and spike proteins.
A novel colorimetric biosensor for detecting SARS-CoV-2 by utilizing the interaction between nucleocapsid antibody and spike proteins.
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DOI：
10.1007/s44164-022-00022-z
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发表时间：
2022
期刊：
影响因子：
--
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中科院分区：
文献类型：
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SARS-CoV-2是一种引起人类严重呼吸道疾病（COVID-19）的大流行性冠状病毒，自2020年初以来导致全球数百万人死亡。该病毒通过位于外壳的刺突（S）蛋白影响人类呼吸道细胞。为了监测SARS-CoV-2的快速传播并减少COVID-19的死亡，早期检测SARS-CoV-2至关重要。本报告描述了一种灵活的比色生物传感器能够检测SARS-CoV-2的S蛋白。该比色传感器由聚氨酯（PU）-聚二乙炔（PDA）复合材料制成，该复合材料被化学官能化以产生SARS-CoV-2的受体分子-核衣壳抗体（抗N）蛋白的结合位点。抗N蛋白与功能化PDA纤维偶联后，PU-PDA-NHS-抗纤维能够在室温下通过从蓝色到红色的比色转变检测SARS-CoV-2的S蛋白。基于PU-PDA纳米纤维的生物传感器是灵活的，重量轻，并且当对S蛋白进行比色检测时不需要电池等电源，这表明可穿戴设备和个人防护设备（如口罩和医用长袍）的传感平台用于实时监测病毒感染和污染。可穿戴生物传感器可以为大规模监控技术提供强大动力，以对抗COVID-19大流行。在线版本包含补充材料，可通过10.1007/s44164-022-00022-z获得。
SARS-CoV-2 is a pandemic coronavirus that causes severe respiratory disease (COVID-19) in humans and is responsible for millions of deaths around the world since early 2020. The virus affects the human respiratory cells through its spike (S) proteins located at the outer shell. To monitor the rapid spreading of SARS-CoV-2 and to reduce the deaths from the COVID-19, early detection of SARS-CoV-2 is of utmost necessity. This report describes a flexible colorimetric biosensor capable of detecting the S protein of SARS-CoV-2. The colorimetric biosensor is made of polyurethane (PU)-polydiacetylene (PDA) nanofiber composite that was chemically functionalized to create a binding site for the receptor molecule—nucleocapsid antibody (anti-N) protein of SARS-CoV-2. After the anti-N protein conjugation to the functionalized PDA fibers, the PU-PDA-NHS-anti fiber was able to detect the S protein of SARS-CoV-2 at room temperature via a colorimetric transition from blue to red. The PU-PDA nanofiber-based biosensors are flexible and lightweight and do not require a power supply such as a battery when the colorimetric detection to S protein occurs, suggesting a sensing platform of wearable devices and personal protective equipment such as face masks and medical gowns for real-time monitoring of virus contraction and contamination. The wearable biosensors could significantly power mass surveillance technologies to fight against the COVID-19 pandemic. The online version contains supplementary material available at 10.1007/s44164-022-00022-z.
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DOI：
10.3390/v13020243
发表时间：
2021-02-04
期刊：
Viruses
影响因子：
--
作者：
Troyano-Hernáez P;Reinosa R;Holguín Á
通讯作者：
Holguín Á
影响因子：
9.5
作者：
Soto, Fernando;Ozen, Mehmet Ozgun;Demirci, Utkan
通讯作者：
Demirci, Utkan
影响因子：
4.1
作者：
Yapor JP;Alharby A;Gentry-Weeks C;Reynolds MM;Alam AKMM;Li YV
通讯作者：
Li YV
影响因子：
10.8
作者：
Jing, Z;Xu, XY;Jing, XB
通讯作者：
Jing, XB
影响因子：
3.4
作者：
Alam, A. K. M. Mashud;Yapor, Janet P.;Li, Yan Vivian
通讯作者：
Li, Yan Vivian