Protective Effects of Parkia biglobosa Protein Isolate on Streptozotocin-Induced Hepatic Damage and Oxidative Stress in Diabetic Male Rats.
Protective Effects of Parkia biglobosa Protein Isolate on Streptozotocin-Induced Hepatic Damage and Oxidative Stress in Diabetic Male Rats.
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DOI：
10.3390/molecules22101654
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发表时间：
2017-10-02
期刊：
影响因子：
--
通讯作者：
Kappo AP
中科院分区：
文献类型：
--
作者：
Ogunyinka BI;Oyinloye BE;Osunsanmi FO;Opoku AR;Kappo AP
本研究旨在探讨双叶百合种子蛋白分离物(PBPI)对链脲佐菌素诱导的雄性糖尿病大鼠肝损伤和氧化应激的可能保护作用。在动物实验之前，记录了PBPI的高效液相指纹图谱。以链脲佐菌素(STZ；60 mg/kg体重)单次腹腔注射诱导大鼠糖尿病。糖尿病大鼠每日口服PBPI(200或400 mg/kg体重)或胰岛素(5U/kg，ip)。已经28天了。以丙二醛、谷草转氨酶、总蛋白、总谷胱甘肽、谷胱甘肽S转移酶、超氧化物歧化酶、过氧化氢酶、白介素6活性等生化指标评价保护程度。取肝组织切片进行组织学检查。PBPI的高效液相指纹图谱显示有11个明显的峰；试验剂量的PBPI显著降低STZ诱导的血清IL-6、ALT和AST水平以及肝脏TBARS水平。肝脏抗氧化剂(总GSH、GST、SOD、CAT)和总蛋白明显恢复，并呈剂量依赖关系。组织病理学结果有力地支持了PBPI的保护作用。这些结果提示，PBPI可通过减轻STZ所致的动物模型肝损伤和氧化应激而发挥保护作用，其机制可能与其抗炎和抗氧化作用有关。
This study sought to investigate the possible protective role of Parkia biglobosa seed protein isolate (PBPi) against streptozotocin-induced hepatic damage and oxidative stress in diabetic male rats. Prior to animal experiments, a HPLC fingerprint of PBPi was recorded. Diabetes was induced in rats by a single intraperitoneal injection of streptozotocin (STZ; 60 mg/kg body weight). Diabetic rats were orally treated daily with PBPi (200 or 400 mg/kg body weight) or insulin (5 U/kg, i.p.) for 28 days. The degree of protection was evaluated using biochemical parameters such as malondialdehyde (MDA) levels, serum transaminases (ALT and AST), total protein, total glutathione (Total GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), and interleukin-6 (IL-6) activities. Histology of liver sections was also performed. The HPLC fingerprint of PBPi revealed eleven distinct peaks; PBPi at tested doses significantly attenuates STZ-induced elevated levels of serum IL-6, ALT and AST; and hepatic TBARS levels. Hepatic antioxidants (Total GSH, GST, SOD, CAT) as well as total protein were markedly restored in a dose-dependent manner. Histopathological results strongly support the protective role of PBPi. These results suggest PBPi could confer protection by ameliorating hepatic damage and oxidative stress caused by STZ in animal model possibly via its anti-inflammatory and antioxidant properties.
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影响因子：
3.1
作者：
Murali, Ramakrishnan;Karthikeyan, Arumugam;Saravanan, Ramalingam
通讯作者：
Saravanan, Ramalingam
影响因子：
4.5
作者：
Kuate D;Kengne AP;Biapa CP;Azantsa BG;Abdul Manan Bin Wan Muda W
通讯作者：
Abdul Manan Bin Wan Muda W
影响因子：
--
作者：
Adeyemi DO;Ukwenya VO;Obuotor EM;Adewole SO
通讯作者：
Adewole SO
影响因子：
3.2
作者：
Deshpande, Anjali D.;Harris-Hayes, Marcie;Schootman, Mario
通讯作者：
Schootman, Mario
影响因子：
1.7
作者：
Balamurugan, Karuppasamy;Nishanthini, Antony;Mohan, Veerabahu Ramasamy
通讯作者：
Mohan, Veerabahu Ramasamy