Biomimetic polymer brushes containing tethered acetylcholine analogs for protein and hippocampal neuronal cell patterning.
Biomimetic polymer brushes containing tethered acetylcholine analogs for protein and hippocampal neuronal cell patterning.
复制标题
DOI：
10.1021/bm301785b
复制
发表时间：
2013-02-11
影响因子：
6.2
通讯作者：
Ober CK
中科院分区：
文献类型：
--
作者：
Zhou Z;Yu P;Geller HM;Ober CK
本文描述了一种通过使用空间定义的聚合物刷图案通过化学和地形线索来控制神经元细胞粘附和分化的方法。首先，采用表面引发原子转移自由基聚合（SI-ATRP）的“生长”方法制备了仿生甲基丙烯酸酯聚合物刷，其含有甲基丙烯酸二甲氨基乙酯（DMAEMA）形式的束缚神经递质乙酰胆碱功能或游离羟基封端的聚（乙二醇）（PEG）单元。 反应。通过各种技术对所得刷子的表面特性进行了彻底的表征，并且刷子表面上的海马神经元细胞培养物表现出的细胞活力和分化能力与常用的聚-L-赖氨酸涂层玻璃盖玻片上的细胞活力和分化能力相当，甚至更好。然后通过 UV 光刻技术对聚合物刷进行图案化，以提供专门设计的具有不同尺寸（从 2 µm 到 200 µm 不等）和方向（水平和垂直）的表面特征。对刷子图案的蛋白质吸收实验和海马神经元细胞培养测试表明，蛋白质和神经元都可以粘附在这些图案上，因此受到这些图案的引导。这些结果还表明，由于其独特的化学成分和明确的性质，所开发的聚合物刷可能在细胞材料相互作用研究和神经组织工程中找到许多潜在的应用。
This paper describes a method to control neuronal cell adhesion and differentiation with both chemical and topographic cues by using a spatially defined polymer brush pattern. First, biomimetic methacrylate polymer brushes containing tethered neurotransmitter acetylcholine functionalities in the form of dimethylaminoethyl methacrylate (DMAEMA), or free hydroxyl-terminated poly(ethylene glycol) (PEG) units were prepared using the “grown from” method through surface-initiated atom transfer radical polymerization (SI-ATRP) reactions. The surface properties of the resulting brushes were thoroughly characterized with various techniques and hippocampal neuronal cell culture on the brush surfaces exhibit cell viability and differentiation comparable to, or even better than, those on commonly used poly-L-lysine coated glass coverslips. The polymer brushes were then patterned via UV photolithography techniques to provide specially designed surface features with different sizes (varying from 2 µm to 200 µm) and orientations (horizontal and vertical). Protein absorption experiments and hippocampal neuronal cell culture tests on the brush patterns showed that both protein and neurons can adhere to the patterns and therefore be guided by such patterns. These results also demonstrate that, because of their unique chemical composition and well-defined nature, the developed polymer brushes may find many potential applications in cell-material interactions studies and neural tissue engineering.
登录
查看更多内容
影响因子：
29.4
作者：
Gumera, Christiane B.;Wang, Yadong
通讯作者：
Wang, Yadong
影响因子：
14
作者：
Schneider, GB;English, A;Keller, J
通讯作者：
Keller, J
影响因子：
4.6
作者：
Branch, DW;Wheeler, BC;Leckband, DE
通讯作者：
Leckband, DE
影响因子：
14
作者：
Ruiz, Ana;Buzanska, Leonora;Ross, Francois
通讯作者：
Ross, Francois
乙酰胆碱样仿生聚合物对神经元生长的影响
影响因子：
14
作者：
Tu, Qin;Li, Li;Wang, Jinyi
通讯作者：
Wang, Jinyi