Fabrication of polylactic acid (PLA)-based porous scaffold through the combination of traditional bio-fabrication and 3D printing technology for bone regeneration.
Fabrication of polylactic acid (PLA)-based porous scaffold through the combination of traditional bio-fabrication and 3D printing technology for bone regeneration.
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DOI：
10.1016/j.colsurfb.2020.111420
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Yu X
中科院分区：
文献类型：
--
作者：
Zhou X;Zhou G;Junka R;Chang N;Anwar A;Wang H;Yu X
人工骨具有良好的生物降解性、可定制的尺寸和足够的力学性能等优点，在骨组织再生中可以促进细胞增殖和分化。3D打印是一种精细的方法，与传统的生物制造方法相比，它赋予支架卓越的可控性和可重复性。然而，打印分辨率的限制使得难以制备具有高孔隙率和分层结构的骨缺损替代物。在这项研究中，我们利用聚乳酸（PLA）作为打印材料，并开发了一种智能策略，将联合收割机3D打印技术与生物制造方法相结合。打印多孔平面支架，然后卷起成具有可调节孔径和孔隙率的螺旋结构。分别用体视显微镜和扫描电镜观察人工支架的形貌特征。多孔螺旋支架在一组力学测试中表现出良好的力学性能。随后，将人胎儿成骨细胞（hFOB）在多孔螺旋支架及其对照组上培养共28天。通过MTS分析、碱性磷酸酶（ALP）测定和茜素红S（ARS）染色分析细胞增殖、成骨分化和一定时间后的矿物质沉积。结果表明，与其他两种支架相比，螺旋形多孔支架具有更大的表面积和更好的内部多孔网络连接，可以显著改善细胞的空间隔室，促进细胞的生长和分化。多孔螺旋支架在大体积骨缺损的再生中具有广泛的应用前景。
Artificial bone grafts possess the advantages of good biodegradability, customizable dimensions, and sufficient mechanical properties, which can promote cell proliferation and differentiation in bone tissue regeneration. 3D printing is a delicate approach that endows the scaffolds with excellent controllability and repeatability when compared with conventional bio-fabrication methods. However, the limitation of printing resolution somehow makes it difficult to prepare bone defect substitution with high porosity and hierarchical construct. In this study, we utilized polylactic acid (PLA) as printing materials and developed a smart strategy to combine 3D printing technology with bio-fabrication methods. A porous planar scaffold was printed and then rolled up into a spiral structure with adjustable pore size and porosity. The topographic features and morphology of the artificial scaffolds were examined through stereomicroscope and SEM, respectively. The porous spiral scaffold presented good mechanical properties in a set of mechanical testing. Later, the human fetal osteoblasts (hFOB) were cultured on the porous spiral scaffold and its control groups for a total of 28 days. The MTS analysis, alkaline phosphatase (ALP) assay, and alizarin red S (ARS) staining were used to analyze the cell proliferation, osteogenic differentiation, and mineral deposition after a certain period of time. The results indicated that compared with the other two scaffolds, the porous spiral scaffold with larger surface area and better interconnections between internal porous networks could significantly improve the spatial cell compartment and promote cell growth and differentiation. The porous spiral scaffold may see versatile applications in large-volume bone defects regeneration.
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影响因子：
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发表时间：
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发表时间：
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影响因子：
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