Maternal transfer and protective role of the alternative complement components in zebrafish Danio rerio.
Maternal transfer and protective role of the alternative complement components in zebrafish Danio rerio.
复制标题
斑马鱼斑马鱼替代补体成分的母体转移和保护作用。
DOI：
10.1371/journal.pone.0004498
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Wang, Guangfeng
中科院分区：
文献类型：
--
作者：
Wang, Zhiping;Zhang, Shicui;Tong, Zhou;Li, Lei;Wang, Guangfeng
大多数鱼类的胚胎在外部发育，并暴露在充满潜在病原体的水生环境中，而它们很少或只有有限的能力来产生有效的保护性反应。鱼类胚胎如何在病原体攻击中存活仍然没有明确的定义。在这里，我们证明了雌性斑马鱼与福尔马林杀死的嗜水气单胞菌的母体免疫导致C3和Bf含量显着增加，在母亲，在后代相应的上升，并诱导显着增加在母亲和后代的溶血活性。此外，来自免疫母体的胚胎对A.用抗C_3和Bf抗体阻断C_3和Bf的活性，使其对A.你好这些结果清楚地表明，斑马鱼胚胎对A。通过旁路途径运作的补体系统的母体转移免疫可以实现免疫应答。这似乎是第一份报告提供体内证据的替代补体成分在斑马鱼早期胚胎中的保护作用，为深入了解鱼类中其他母体转移因子的体内功能铺平了道路。
Embryos of most fish develop externally and are exposed to an aquatic environment full of potential pathogens, whereas they have little or only limited ability to mount an efficient and protective response. How fish embryos survive pathogenic attacks remains poorly defined. Here we demonstrate that the maternal immunization of female zebrafish with formalin-killed Aeromonas hydrophila causes a significant increase in C3 and Bf contents in the mother, a corresponding rise in the offspring, and induces a remarkable increase in the hemolytic activities in both the mother and offspring. In addition, the embryos derived from the immunized mother are significantly more tolerant to A. hydrophila challenge than those from the unimmunized fish, and blocking C3 and Bf activities by injection of the antibodies against C3 and Bf into the embryos render them more susceptible to A. hydrophila. These results clearly show that the protection of zebrafish embryos against A. hydrophila can be achieved by the maternally-transferred immunity of the complement system operating via the alternative pathway. This appears to be the first report providing in vivo evidences for the protective role of the alternative complement components in the early embryos of zebrafish, paving the way for insights into the in vivo function of other maternally-transferred factors in fish.
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