Molecular characterization of Cryptosporidium spp. and Giardia duodenalis from yaks in the central western region of China.
Molecular characterization of Cryptosporidium spp. and Giardia duodenalis from yaks in the central western region of China.
复制标题
隐孢子虫属的分子特征。
DOI：
10.1186/s12866-015-0446-0
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发表时间：
2015-05-21
期刊：
影响因子：
4.2
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Qi M;Cai J;Wang R;Li J;Jian F;Huang J;Zhou H;Zhang L
背景：隐孢子虫属。和贾第鞭毛虫是世界范围内人类和动物肠道疾病的重要原因，并且对动物在这些原生动物的机械传播中的作用越来越感兴趣。为了研究牦牛在这一过程中的作用，我们检测了隐孢子虫和G。结果：从中国中西部地区9个县（市、区）的牦牛粪便中共采集到545份标本。隐孢子虫的流行。和G.膀胱癌分别为4.0%（22/545）和6.0%（16/545）。隐孢子虫与G.在4份标本中也检测到了尿道炎。这两种原虫的流行率在一些年龄组之间差异很大，1岁以下动物的感染率较高。通过对隐孢子虫小亚基rRNA基因的序列分析，确定该虫为隐孢子虫C. parvum（n = 12）、C. bovis（n = 6）、C. ryanae（n = 3）和C.泛素（n = 1）。对5株念珠菌的60 kDa糖蛋白（gp 60）基因进行分型。parvum分离物鉴定为IId，其中3个分离物鉴定为IIdA 15 G1，1个鉴定为IIdA 18 G1，1个鉴定为IIdA 19 G1。一个C。泛素分离物被鉴定为VIIa亚型。在G. 16个菌株在SSU rRNA基因上被鉴定为E组合。在G.结论：在大肠杆菌中存在C. IIdA 15 G1、IIdA 18 G1和IIdA 19 G1亚型进一步证实了C. parvum IId亚型。牦牛可能是人畜共患隐孢子虫病的一个传染源。牦牛体内的寄生虫。本研究结果为进一步研究G.牦牛体内的寄生虫。
Background:Cryptosporidium spp. and Giardia duodenalis are important causes of diarrheal diseases in humans and animals worldwide, and there is an increased interest in the role of animals in the mechanical transmission of these protozoa. To examine the role of yaks in this process, we examined the occurrence and genotypes of Cryptosporidium and G. duodenalis in yaks in western China.Results:A total of 545 fecal specimens were collected from yaks from nine different counties in the central western region of China. The prevalence for Cryptosporidium spp. and G. duodenalis were 4.0 % (22/545) and 6.0 % (16/545), respectively. Mixed infections of Cryptosporidium and G. duodenalis were also detected in four specimens. The prevalence of both protozoa differed significantly between some age groups, with higher rates of infection in animals < 1 year old. Sequence analysis of the small subunit rRNA (SSU rRNA) gene of the Cryptosporidium isolates identified the species as C. parvum (n = 12), C. bovis (n = 6), C. ryanae (n = 3), and C. ubiquitum (n = 1). Genotyping based on 60-kDa glycoprotein (gp60) gene from five C. parvum isolates identified all as IId with three isolates identified as IIdA15G1, one as IIdA18G1, and one as IIdA19G1. One C. ubiquitum isolate was identified as subtype VIIa. Amongst the G. duodenalis isolates, 16 were identified as assemblage E at the SSU rRNA gene. Four novel glutamate dehydrogenase (gdh) subtypes and two triosephosphate isomerase (tpi) subtypes were found amongst the G. duodenalis assemblage E isolates.Conclusions:The presence of C. parvum subtype IIdA15G1, IIdA18G1, and IIdA19G1 isolates further confirms the dominance of the C. parvum IId subtypes in China. These findings also indicate that yaks may be a source of zoonotic Cryptosporidium infection, and this is the first report of G. duodenalis in yaks. The data presented here provides the basis for further genotyping or subtyping studies of G. duodenalis in yaks.
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影响因子：
2.6
作者：
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通讯作者：
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影响因子：
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Li N;Xiao L;Alderisio K;Elwin K;Cebelinski E;Chalmers R;Santin M;Fayer R;Kvac M;Ryan U;Sak B;Stanko M;Guo Y;Wang L;Zhang L;Cai J;Roellig D;Feng Y
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影响因子：
2.6
作者：
Appelbee, AJ;Frederick, LM;Olson, ME
通讯作者：
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通过废水中寄生虫的基因分型和分型对隐孢子虫属、十二指肠贾第鞭毛虫和比氏肠细胞虫进行分子监测
影响因子：
3.8
作者：
Li N;Xiao L;Wang L;Zhao S;Zhao X;Duan L;Guo M;Liu L;Feng Y
通讯作者：
Feng Y
影响因子：
3.7
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Liu A;Yang F;Shen Y;Zhang W;Wang R;Zhao W;Zhang L;Ling H;Cao J
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