Evaluation of antiviral activities of Houttuynia cordata Thunb. extract, quercetin, quercetrin and cinanserin on murine coronavirus and dengue virus infection.
Evaluation of antiviral activities of Houttuynia cordata Thunb. extract, quercetin, quercetrin and cinanserin on murine coronavirus and dengue virus infection.
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DOI：
10.1016/j.apjtm.2015.12.002
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发表时间：
2016-01
影响因子：
3.1
通讯作者：
Chow VT
中科院分区：
文献类型：
--
作者：
Chiow KH;Phoon MC;Putti T;Tan BK;Chow VT
目的：评价鱼腥草乙酸乙酯部位的体外活性。抗小鼠冠状病毒和登革病毒(DENV)的三种黄酮类成分(栎皮素、栎皮苷和芦丁)。采用小鼠肝炎病毒(MHV)和2型登革热病毒(DENV-2)的病毒中和试验，评价了不同浓度的黄连提取物和黄酮类成分的抗病毒活性。还对盐酸桂色林进行了抗MHV试验。用C57BL/6小鼠进行急性经口毒性试验。海参EA组分对病毒的侵染性抑制达6d，而盐酸桂色林仅有2d的抑制作用，在病毒吸附阶段前加入的海参EA组分对MHV的半数抑制浓度(IC_(50))为0.98g/mL，对DENV-2的半数抑制浓度(IC50)为7.50g/mL，无细胞毒性。喂饲高达2000 mg/kg的EA组小鼠未出现任何急性毒性迹象，主要器官的组织学特征正常。某些黄酮类化合物表现出相对较弱的抗病毒活性，尤其是槲皮素，它可以同时抑制MHV和DENV-2。其次是槲皮苷，对DENV-2病毒有抑制作用，但对MHV无抑制作用，芦丁对这两种病毒均无抑制作用。与槲皮苷联合应用，可增强抗DENV-2的活性，降低细胞毒作用。然而，黄酮类化合物的协同作用仍然弱于EA组分。海参中的化合物有助于EA组分优越的抗病毒效果，因为EA组分在体外缺乏细胞毒性，在体内缺乏急性毒性。H.Cordata在开发抗冠状病毒和登革热感染的抗病毒药物方面有很大潜力。
To evaluate the in vitro activities of the ethyl acetate (EA) fraction of Houttuynia cordata (H. cordata) Thunb. (Saururaceae) and three of its constituent flavonoids (quercetin, quercitrin and rutin) against murine coronavirus and dengue virus (DENV). The antiviral activities of various concentrations of the EA fraction of H. cordata and flavonoids were assessed using virus neutralization tests against mouse hepatitis virus (MHV) and DENV type 2 (DENV-2). Cinanserin hydrochloride was also tested against MHV. The EA fraction of H. cordata was tested for acute oral toxicity in C57BL/6 mice. The EA fraction of H. cordata inhibited viral infectivity up to 6 d. Cinanserin hydrochloride was able to inhibit MHV for only 2 d. The 50% inhibitory concentrations (IC50) of the EA fraction of H. cordata added before the viral adsorption stage were 0.98 μg/mL for MHV and 7.50 μg/mL for DENV-2 with absence of cytotoxicity. The mice fed with the EA fraction up to 2 000 mg/kg did not induce any signs of acute toxicity, with normal histological features of major organs. Certain flavonoids exhibited comparatively weaker antiviral activity, notably quercetin which could inhibit both MHV and DENV-2. This was followed by quercitrin which could inhibit DENV-2 but not MHV, whereas rutin did not exert any inhibitory effect on either virus. When quercetin was combined with quercitrin, enhancement of anti-DENV-2 activity and reduced cytotoxicity were observed. However, the synergistic efficacy of the flavonoid combination was still less than that of the EA fraction. The compounds in H. cordata contribute to the superior antiviral efficacy of the EA fraction which lacked cytotoxicity in vitro and acute toxicity in vivo. H. cordata has much potential for the development of antiviral agents against coronavirus and dengue infections.
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影响因子：
5.4
作者：
Lamien, CE;Meda, A;Viljoen, GJ
通讯作者：
Viljoen, GJ
影响因子：
4
作者：
Leardkamolkarn, Vijittra;Sirigulpanit, Wipawan;Sripanidkulchai, Bungorn
通讯作者：
Sripanidkulchai, Bungorn
影响因子：
2.7
作者：
HAYASHI, K;KAMIYA, M;HAYASHI, T
通讯作者：
HAYASHI, T
影响因子：
7.6
作者：
Chen X;Wang Z;Yang Z;Wang J;Xu Y;Tan RX;Li E
通讯作者：
Li E
影响因子：
7.6
作者：
Song, JM;Lee, KH;Seong, BL
通讯作者：
Seong, BL