The anti-porcine parvovirus activity of nanometer propolis flavone and propolis flavone in vitro and in vivo.
The anti-porcine parvovirus activity of nanometer propolis flavone and propolis flavone in vitro and in vivo.
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纳米蜂胶黄酮及蜂胶黄酮体内外抗猪细小病毒活性
DOI：
10.1155/2015/472876
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Fan Y
中科院分区：
文献类型：
--
作者：
Ma X;Guo Z;Shen Z;Liu Y;Wang J;Fan Y
目标.本研究旨在评价纳米葛根黄酮（NPF）对猪细小病毒（PPV）的体内外抑制作用。方法.在体外实验中，观察了NPF加药前后及与PPV混合后同时加药对PPV细胞感染性的影响。在豚鼠体内，观察了NPF的抗PPV作用。结果结果表明，NPF对PPV感染猪肾-（PK-）15细胞有明显的抑制作用，且NPF在预加药模式下的抑制作用最强。NPF高、中剂量组能显著抑制PPV在肺、性腺、血液和脾脏中的复制，减轻PPV对豚鼠体重的影响，提高血清中PPV的血凝抑制（HI）。此外，还能提高PPV攻击后血清中IL-2、IL-6的含量。结论结果表明，NPF能显著提高PF的抗PPV活性，且以高浓度的效果最好。因此，NPF有望开发成为一种新型的抗病毒药物。
Objectives. The present study was conducted to evaluate the activity of nanometer propolis flavone (NPF) on inhibiting porcine parvovirus (PPV) in vitro and in vivo. Methods. In vitro, the effect of NPF on cellular infectivity of PPV was carried out before and after adding drug and simultaneous adding and PPV after being mixed. In vivo, the anti-PPV effect of NPF in guinea pigs was performed. Results. The results showed that NPF could significantly inhibit PPV infecting porcine kidney- (PK-) 15 cells compared with propolis flavone (PF), and the activity of NPF was the best in preadding drug pattern. NPF at high and medium doses was able to observably restrain PPV copying in lung, gonad, blood, and spleen, decrease the impact of PPV on weight of guinea pigs, and improve hemagglutination inhibition (HI) of PPV in serum. In addition, it could also increase the contents of IL-2 and IL-6 in serum after PPV challenge. Conclusion. These results indicated that NPF could significantly improve the anti-PPV activity of PF, and its high concentration possessed the best efficacy. Therefore, NPF would be expected to be exploited into a new-style antiviral drug.
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DOI：
10.1016/j.ijbiomac.2012.04.028
发表时间：
2012-10
影响因子：
8.2
作者：
Yuqing Z;Zhan'ao S;Jiaguo L;Deyun W;Baokang Z;Fang Y;Yunpeng F;Dan L;Xu L;Cui L;Yuanliang H
通讯作者：
Yuanliang H
硫酸化修饰可增强香菇多糖的佐剂作用，提高ND疫苗的免疫效果
影响因子：
5.5
作者：
Guo, Zhenhuan;Hu, Yuanliang;Liu, Ping
通讯作者：
Liu, Ping
影响因子：
2.5
作者：
Segales, Joaquim;Allan, Gordon M.;Domingo, Mariano
通讯作者：
Domingo, Mariano
影响因子：
3.3
作者：
JOO, HS;MOLITOR, TW;LEMAN, AD
通讯作者：
LEMAN, AD
影响因子：
5.5
作者：
Fischer, Geferson;Conceicao, Fabricio Rochedo;Vidor, Telmo
通讯作者：
Vidor, Telmo