Detection of a single identical cytomegalovirus (CMV) strain in recently seroconverted young women.
Detection of a single identical cytomegalovirus (CMV) strain in recently seroconverted young women.
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DOI：
10.1371/journal.pone.0015949
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发表时间：
2011-01-10
期刊：
影响因子：
3.7
通讯作者：
Arav-Boger R
中科院分区：
文献类型：
--
作者：
Murthy S;Hayward GS;Wheelan S;Forman MS;Ahn JH;Pass RF;Arav-Boger R
在免疫功能低下的宿主中，多株CMV感染是常见的，但其在正常宿主中的发生还没有得到很好的研究。我们纵向分析了在参加CMV糖蛋白B(GB)疫苗试验时感染CMV的妇女的CMV毒株。在从血清转换中收集的样本中进行了四个变量基因的测序，并在此后长达34个月。来自53名女性的199个培养菌株和19个女性子组的65个原始体液被测序。51名女性感染了一种菌株，每个人都没有遗传漂移的证据；只有两名女性感染了多个菌株。菌株间的遗传变异随测序遗传位点数的增加而增加。然而，根据所有四个可变基因的测序，53名女性中有13人被证明感染了相同的CMV毒株。巨细胞病毒疫苗不会改变菌株间的遗传多样性程度。健康女性的原发CMV感染几乎总是涉及一种毒株的脱落，这种毒株在一段时间内保持稳定。CMVgB-1疫苗株免疫对特定毒株没有选择性。尽管75%的女性携带独特的菌株，或只与另一名女性共用一种菌株，但25%的女性共享单一的共同菌株，这表明这种具有特定遗传位点组合的优势菌株在这个大城市地区是有利的。
Infection with multiple CMV strains is common in immunocompromised hosts, but its occurrence in normal hosts has not been well-studied. We analyzed CMV strains longitudinally in women who acquired CMV while enrolled in a CMV glycoprotein B (gB) vaccine trial. Sequencing of four variable genes was performed in samples collected from seroconversion and up to 34 months thereafter. 199 cultured isolates from 53 women and 65 original fluids from a subset of 19 women were sequenced. 51 women were infected with one strain each without evidence for genetic drift; only two women shed multiple strains. Genetic variability among strains increased with the number of sequenced genetic loci. Nevertheless, 13 of 53 women proved to be infected with an identical CMV strain based on sequencing at all four variable genes. CMV vaccine did not alter the degree of genetic diversity amongst strains. Primary CMV infection in healthy women nearly always involves shedding of one strain that remains stable over time. Immunization with CMVgB-1 vaccine strain is not selective against specific strains. Although 75% of women harbored their unique strain, or a strain shared with only one other woman, 25% shared a single common strain, suggesting that this predominant strain with a particular combination of genetic loci is advantageous in this large urban area.
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影响因子：
5.1
作者：
Bale, JF;Petheram, SJ;Murph, JR
通讯作者：
Murph, JR
影响因子：
6.4
作者：
CHANDLER, SH;HANDSFIELD, HH;MCDOUGALL, JK
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MCDOUGALL, JK
影响因子：
6.4
作者：
Arav-Boger, R;Willoughby, RE;Hayward, GS
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Hayward, GS
影响因子：
3.8
作者：
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通讯作者：
Davison, AJ
影响因子：
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作者：
Revello, Maria Grazia;Campanini, Giulia;Gerna, Giuseppe
通讯作者：
Gerna, Giuseppe