Collagenous bone matrix-induced endochondral ossification hemopoiesis.
Collagenous bone matrix-induced endochondral ossification hemopoiesis.
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DOI：
10.1083/jcb.69.3.557
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发表时间：
1976-06
期刊：
影响因子：
--
通讯作者：
Anderson WA
中科院分区：
文献类型：
--
作者：
Reddi AH;Anderson WA
将大鼠骨干骨胶原基质移植到皮下部位，通过软骨内序列形成新骨。功能性骨髓在新形成的听骨中发育。第一天，植入的基质是一个离散的集合体，含有纤维蛋白凝块和多形核白细胞。到第3天，白细胞消失，随后成纤维细胞表面迁移并紧密贴附于胶原基质。这种最初的基质-膜相互作用最终导致成纤维细胞分化为软骨母细胞和成骨细胞。肥大的软骨细胞钙化和新骨形成与碱性磷酸酶活性和45Ca掺入增加有关。第9天毛细血管长入导致软骨溶解和成骨。破骨细胞进一步重塑骨小梁，形成松质骨的骨小骨。随之而来的是血管外成血细胞岛的出现，并分化为具有功能的骨髓，在听骨中含有红系和粒系成分以及巨核细胞。通过~(59)Fe掺入蛋白结合的亚铁血红素来监测诱导骨髓中红细胞生成的开始和维持。这些发现暗示了细胞外胶原基质在细胞分化中的作用。
Transplantation of collagenous matrix from the rat diaphyseal bone to subcutaneous sites resulted in new bone formation by an endochondral sequence. Functional bone marrow develops within the newly formed ossicle. On day 1, the implanted matrix was a discrete conglomerate with fibrin clot and polymorphonuclear leukocytes. By day 3, the leukocytes disappeared, and this event was followed by migration and close apposition of fibroblast cell surface to the collagenous matrix. This initial matrix-membrane interaction culminated in differentiation of fibroblasts to chondroblasts and osteoblasts. The calcification of the hypertrophied chondrocytes and new bone formation were correlated with increased alkaline phosphatase activity and 45Ca incorporation. The ingrowth of capillaries on day 9 resulted in chondrolysis and osteogenesis. Further remodelling of bony trabeculae by osteoclasts resulted in an ossicle of cancellous bone. This was followed by emergence of extravascular islands of hemocytoblasts and their differentiation into functional bone marrow with erythropoietic and granulopoietic elements and megakaryocytes in the ossicle. The onset and maintenance of erythropoiesis in the induced bone marrow were monitored by 59Fe incorporation into protein-bound heme. These findings imply a role for extracellular collagenous matrix in cell differentiation.
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DOI：
10.1073/pnas.71.5.1648
发表时间：
1974-01-01
影响因子：
11.1
作者：
REDDI, AH;HUGGINS, CB
通讯作者：
HUGGINS, CB
影响因子：
3.2
作者：
GRAHAM, RC;KARNOVSKY, MJ
通讯作者：
KARNOVSKY, MJ
DOI：
10.1097/00003086-197107000-00010
发表时间：
1971-01-01
影响因子：
4.2
作者：
Bonucci, E
通讯作者：
Bonucci, E
影响因子：
--
作者：
CRISSMAN, RS;LOW, FN
通讯作者：
LOW, FN
影响因子：
4.1
作者：
Robison, R
通讯作者：
Robison, R