Elucidation of the miR164c-Guided Gene/Protein Interaction Network Controlling Seed Vigor in Rice.
Elucidation of the miR164c-Guided Gene/Protein Interaction Network Controlling Seed Vigor in Rice.
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阐明 miR164c 引导的基因/蛋白质相互作用网络控制水稻种子活力
DOI：
10.3389/fpls.2020.589005
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发表时间：
2020
影响因子：
5.6
通讯作者：
Jiang X
中科院分区：
文献类型：
--
作者：
Huang K;Zhou S;Shen K;Zhou Y;Wang F;Jiang X
microRNAs（miRNAs）在植物生理和代谢的各个方面发挥着重要作用。miR 164 c的表达水平与水稻种子活力呈负相关;然而，miR 164 c调节种子活力的机制仍不清楚。抗衰老能力是种子活力的重要指标。在这里，我们报告了一个miR 164 c引导的基因/蛋白质相互作用网络，调节水稻种子的抗衰老能力。野生型水稻品种“Kasalath”及其转基因衍生物、miR 164 c沉默系（MIM 164 c）和miR 164 c过表达系（OE 164 c）的种子在抗衰老能力方面存在显着差异，基因和蛋白质表达水平也存在显着差异。差异表达的基因或蛋白质显著富集在与种子活力相关的6个代谢功能类别中，包括“胁迫反应”、“内质网（ER）中的蛋白质加工”、“胚胎发育”、“丝氨酸型内肽酶抑制剂”、“能量代谢”和“其他”。正常贮藏条件下种子中与能量代谢、丝氨酸内肽酶和胁迫反应相关的基因或蛋白质表达水平的差异可能与抗衰老能力有关。基因/蛋白质相互作用分析的结果表明，miR 164 c首先靶向PSK 5，然后PSK 5蛋白与泛素相关基因RPS 27 AA相互作用，这同时影响上述6个功能类别的基因/蛋白质。通过实时荧光定量PCR（RT-qPCR）和多反应监测质谱（MRM-MS）分别验证了相互作用网络中一些关键基因和蛋白的表达水平。因此，本研究为调节种子活力的miRNA介导的基因和蛋白质相互作用网络提供了新的见解。
MicroRNAs (miRNAs) play important roles in various aspects of plant physiology and metabolism. The expression level of miR164c is negatively correlated with seed vigor in rice (Oryza sativa L.); however, the mechanism of seed vigor regulation by miR164c remains unknown. Anti-aging capacity is an important indicator of seed vigor. Here, we report an miR164c-guided gene/protein interaction network that regulates the anti-aging ability of rice seeds. Seeds of the wild-type (WT) rice cultivar “Kasalath” and its transgenic derivatives, miR164c-silenced line (MIM164c) and miR164c overexpression line (OE164c), with significant differences in anti-aging capacity, showed significant differences in gene and protein expression levels. The differentially expressed genes (DEGs) or proteins were significantly enriched in six metabolic functional categories related to seed vigor, including “stress response,” “protein processing in endoplasmic reticulum (ER),” “embryo development,” “serine-type endopeptidase inhibitor,” “energy metabolism,” and “other.” Differences in the expression levels of genes or proteins related to energy metabolism, serine endopeptidase, and stress response in seeds under normal storage conditions may be associated with anti-aging capacity. The results of gene/protein interaction analyses suggest that miR164c first targets PSK5, and the PSK5 protein then interacts with the ubiquitin-associated gene RPS27AA, which simultaneously impacts the genes/proteins in the six above-mentioned functional categories. Expression levels of some of the key genes and proteins in the interaction network were verified by real-time fluorescence quantitative PCR (RT-qPCR) and multiple reaction monitoring mass spectrometry (MRM-MS), respectively. Thus, the present study provides new insights into the miRNA-mediated gene and protein interaction network that regulates seed vigor.
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影响因子：
3.4
作者：
Catusse, Julie;Meinhard, Juliane;Job, Dominique
通讯作者：
Job, Dominique
影响因子：
1.4
作者：
Freitas, R. A.;Dias, D. C. F. S.;Jose, I. C.
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DOI：
10.1016/j.bbagrm.2011.05.001
发表时间：
2012-02
影响因子：
4.7
作者：
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Zhu, Jianhua
DOI：
10.1007/978-981-13-1244-1_13
发表时间：
2018-01-01
期刊：
SURVIVAL STRATEGIES IN EXTREME COLD AND DESICCATION: ADAPTATION MECHANISMS AND THEIR APPLICATIONS
影响因子：
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作者：
Ishibashi, Yushi;Yuasa, Takashi;Iwaya-Inoue, Mari
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影响因子：
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作者：
Li, Yan;Qu, Haibin;Zhang, Chunqing
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