Human-origin probiotic cocktail increases short-chain fatty acid production via modulation of mice and human gut microbiome.
Human-origin probiotic cocktail increases short-chain fatty acid production via modulation of mice and human gut microbiome.
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DOI：
10.1038/s41598-018-30114-4
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发表时间：
2018-08-23
影响因子：
4.6
通讯作者：
Yadav H
中科院分区：
文献类型：
--
作者：
Nagpal R;Wang S;Ahmadi S;Hayes J;Gagliano J;Subashchandrabose S;Kitzman DW;Becton T;Read R;Yadav H
肠道细菌产生代谢物，如短链脂肪酸（SCFA;例如，乙酸盐、丙酸盐和丁酸盐）在糖尿病、肥胖症、自身免疫性疾病和癌症患者中经常减少。因此，微生物组调节剂如益生菌可能有助于维持甚至恢复正常的肠道微生物组组成，以有益于宿主健康。在此，我们开发了一种人源益生菌鸡尾酒，其具有调节肠道微生物群以增加天然SCFA产生的能力。根据婴儿肠道来源的乳酸杆菌和肠球菌菌株的分离，表征和安全性验证的强大协议，具有益生菌属性（耐受模拟胃和肠道条件，粘附肠上皮细胞，缺乏潜在的毒力基因，细胞表面疏水性和对常见抗生素的敏感性），我们从总共321株菌株中选择了10株（每属5株）。在小鼠中单次剂量（口服灌胃）以及连续5次剂量的这种10株益生菌混合物调节肠道微生物组并增加SCFA产生（特别是丙酸盐和丁酸盐）。在人类粪便中接种这些益生菌也增加了SCFA的产生，沿着微生物组调节。结果表明，人类来源的益生菌乳酸杆菌和肠球菌可以改善肠道微生物组生态失调，因此可能被证明是一种潜在的治疗方法，用于治疗涉及肠道中SCFA产生减少的疾病。
The gut bacteria producing metabolites like short-chain fatty acids (SCFAs; e.g., acetate, propionate and butyrate), are frequently reduced in Patients with diabetes, obesity, autoimmune disorders, and cancers. Hence, microbiome modulators such as probiotics may be helpful in maintaining or even restoring normal gut microbiome composition to benefit host health. Herein, we developed a human-origin probiotic cocktail with the ability to modulate gut microbiota to increase native SCFA production. Following a robust protocol of isolation, characterization and safety validation of infant gut-origin Lactobacillus and Enterococcus strains with probiotic attributes (tolerance to simulated gastric and intestinal conditions, adherence to intestinal epithelial cells, absence of potential virulence genes, cell-surface hydrophobicity, and susceptibility to common antibiotics), we select 10 strains (5 from each genera) out of total 321 isolates. A single dose (oral gavage) as well as 5 consecutive doses of this 10-strain probiotic cocktail in mice modulates gut microbiome and increases SCFA production (particularly propionate and butyrate). Inoculation of these probiotics in human feces also increases SCFA production along with microbiome modulation. Results indicate that human-origin probiotic lactobacilli and enterococci could ameliorate gut microbiome dysbiosis and hence may prove to be a potential therapy for diseases involving reduced SCFAs production in the gut.
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影响因子：
5.4
作者：
Behnsen, Judith;Deriu, Elisa;Raffatellu, Manuela
通讯作者：
Raffatellu, Manuela
影响因子：
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Bischoff SC;Barbara G;Buurman W;Ockhuizen T;Schulzke JD;Serino M;Tilg H;Watson A;Wells JM
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4.2
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通讯作者：
Versalovic J
影响因子：
3.1
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