Intravenous Infusion of Bone Marrow-Derived Mesenchymal Stem Cells Reduces Erectile Dysfunction Following Cavernous Nerve Injury in Rats.
Intravenous Infusion of Bone Marrow-Derived Mesenchymal Stem Cells Reduces Erectile Dysfunction Following Cavernous Nerve Injury in Rats.
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DOI：
10.1016/j.esxm.2017.10.005
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发表时间：
2018-03
期刊：
影响因子：
2.6
通讯作者：
Honmou O
中科院分区：
文献类型：
--
作者：
Matsuda Y;Sasaki M;Kataoka-Sasaki Y;Takayanagi A;Kobayashi K;Oka S;Nakazaki M;Masumori N;Kocsis JD;Honmou O
静脉预负荷骨髓间充质干细胞（MSCs）可预防或减轻术后勃起功能障碍（J Sex Med 2015; 12:1713-1721）。在本研究中，在大鼠CN损伤模型中评估静脉给药MSCs对术后勃起功能障碍的潜在治疗作用。雄性Sprague-Dawley大鼠电损伤后随机分为2组。在电致CN损伤3小时后静脉输注骨髓源性MSCs （1.0 × 106个细胞，Dulbecco's modified Eagle's培养基1 mL）或载药（Dulbecco's modified Eagle's培养基1 mL）。为了评估勃起功能，我们在骨髓间充质干细胞或载体输注后4周测量海绵内压力。组织学检查观察神经支配和平滑肌萎缩的抑制作用。采用绿色荧光蛋白阳性骨髓源性间充质干细胞进行细胞跟踪。采用实时定量聚合酶链反应研究大鼠盆腔神经节（MPGs）神经营养因子mRNA表达水平。灌注后4周，骨髓源性间充质干细胞组经动脉压校正后的海绵内压下降量和海绵内压曲线下面积均显著低于载药组（P < 0.05）。逆行神经元示踪显示，与载药组相比，MSC组在mpg中有更多的fluorogold阳性神经元。骨髓间充质干细胞组平滑肌与胶原的比值显著高于载药组。利用共聚焦显微镜在mpg和损伤的CNs中检测到绿色荧光蛋白阳性的骨髓来源的MSCs，表明细胞归巢到mpg和损伤的CNs。骨髓间充质干细胞组MPGs中脑源性神经营养因子和胶质细胞源性神经营养因子的表达水平显著高于载药组（P < 0.01）。静脉输注骨髓源性间充质干细胞可能对实验性勃起功能障碍有治疗作用。Matsuda Y, Sasaki M, Kataoka-Sasaki Y，等。静脉输注骨髓间充质干细胞可减少海绵状神经损伤后大鼠勃起功能障碍。性医学2018；6:49-57。
Intravenous preload (delivered before cavernous nerve [CN] injury) of bone marrow–derived mesenchymal stem cells (MSCs) can prevent or decrease postoperative erectile dysfunction (J Sex Med 2015;12:1713–1721). In the present study, the potential therapeutic effects of intravenously administered MSCs on postoperative erectile dysfunction were evaluated in a rat model of CN injury. Male Sprague-Dawley rats were randomized into 2 groups after electric CN injury. Intravenous infusion of bone marrow–derived MSCs (1.0 × 106 cells in Dulbecco's modified Eagle's medium 1 mL) or vehicle (Dulbecco's modified Eagle's medium 1 mL) was performed 3 hours after electrocautery-induced CN injury. To assess erectile function, we measured intracavernous pressure at 4 weeks after MSC or vehicle infusion. Histologic examinations were performed to investigate neuronal innervation and inhibition of smooth muscle atrophy. Green fluorescent protein–positive bone marrow–derived MSCs were used for cell tracking. To investigate mRNA expression levels of neurotrophins in the major pelvic ganglia (MPGs), quantitative real-time polymerase chain reaction was performed. The decrease of intracavernous pressure corrected for arterial pressure and area under the curve of intracavernous pressure in the bone marrow–derived MSC group was significantly lower than that in the vehicle group at 4 weeks after infusion (P < .05). Retrograde neuronal tracing indicated that the MSC group had a larger number of FluoroGold-positive neurons in the MPGs compared with the vehicle group. The ratio of smooth muscle to collagen in the MSC group was significantly higher than in the vehicle group. Green fluorescent protein–positive bone marrow–derived MSCs were detected in the MPGs and injured CNs using confocal microscopy, indicating homing of cells to the MPGs and injured CNs. Brain-derived neurotrophic factor and glial cell-derived neurotrophic factor expression levels in the MPGs were significantly higher in the MSC group than in the vehicle group (P < .01). Intravenous infusion of bone marrow–derived MSCs after CN injury might have therapeutic efficacy in experimental erectile dysfunction. Matsuda Y, Sasaki M, Kataoka-Sasaki Y, et al. Intravenous Infusion of Bone Marrow–Derived Mesenchymal Stem Cells Reduces Erectile Dysfunction Following Cavernous Nerve Injury in Rats. Sex Med 2018;6:49–57.
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