Herbal Compound Songyou Yin and Moderate Swimming Suppress Growth and Metastasis of Liver Cancer by Enhancing Immune Function.
Herbal Compound Songyou Yin and Moderate Swimming Suppress Growth and Metastasis of Liver Cancer by Enhancing Immune Function.
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复方松右饮与适量游泳增强免疫功能抑制肝癌生长和转移
DOI：
10.1177/1534735415622011
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发表时间：
2016-09
影响因子：
2.9
通讯作者：
Tang ZY
中科院分区：
文献类型：
--
作者：
Zhang QB;Meng XT;Jia QA;Bu Y;Ren ZG;Zhang BH;Tang ZY
客观的。在动物模型中，中草药复方松柚饮（SYY）和游泳运动均被证明对肝癌具有预防作用。在这项研究中，我们研究了 SYY 和适度游泳 (MS) 是否通过免疫调节增强抑制肝癌进展的效果。方法。 C57BL/6小鼠移植Hepa1-6鼠肝癌细胞系，并接受SYY单独治疗或SYY联合MS治疗。使用立体荧光显微镜对肺部绿色荧光蛋白（GFP）阳性转移灶进行成像。采用流式细胞术检测外周血中CD4+、CD8+T细胞比例以及外周血、脾脏、肿瘤组织中CD4+CD25+Foxp3+Treg细胞比例。采用ELISA法检测血清中细胞因子转化生长因子(TGF)-β1水平。结果。 SYY 联合 MS 显着抑制肝癌的生长和肺转移，并延长荷瘤小鼠的生存期。 SYY联合MS显着提高外周血CD4/CD8比值，降低血清TGF-β1水平以及外周血、脾脏和肿瘤组织中Treg细胞的比例。联合干预的效果明显优于单独使用SYY或MS。结论。 SYY与MS联合应用，通过增强免疫力，增强抑制肝癌生长和转移的作用。
Objective. Both the Chinese herbal compound Songyou Yin (SYY) and swimming exercise have been shown to have protective effects against liver cancer in animal models. In this study, we investigated whether SYY and moderate swimming (MS) have enhanced effect on suppressing progression of liver cancer by immunomodulation. Methods. C57BL/6 mice were transplanted with Hepa1-6 murine liver cancer cell lines and received treatment with SYY alone or SYY combined with MS. The green fluorescent protein (GFP)-positive metastatic foci in lungs were imaged with a stereoscopic fluorescence microscope. Flow cytometry was used to test the proportion of CD4 +, CD8 + T cells in peripheral blood and the proportions of CD4 + CD25 + Foxp3 + Treg cells in peripheral blood, spleen, and tumor tissues. Cytokine transforming growth factor (TGF)-β1 level in serum was detected by ELISA. Results. SYY plus MS significantly suppressed the growth and lung metastasis of liver cancer and prolonged survival in tumor-burdened mice. SYY plus MS markedly raised the CD4 to CD8 ratio in peripheral blood and lowered the serum TGF-β1 level and the proportions of Treg cells in peripheral blood, spleen, and tumor tissue. The effects of the combined intervention were significantly superior to SYY or MS alone. Conclusion. The combined application of SYY and MS exerted an enhanced effect on suppressing growth and metastasis of liver cancer by strengthening immunity.
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DOI：
10.1155/2012/908601
发表时间：
2012
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Jia QA;Ren ZG;Bu Y;Wang ZM;Zhang QB;Liang L;Jiang XM;Zhang QB;Tang ZY
通讯作者：
Tang ZY
影响因子：
3.3
作者：
Murphy, EA;Davis, JM;Ghaffar, A
通讯作者：
Ghaffar, A
影响因子：
6.4
作者：
Hara, T;Nishimura, H;Yoshikai, Y
通讯作者：
Yoshikai, Y
影响因子：
2.4
作者：
Abdalla, Douglas R.;Murta, Eddie F. C.;Michelin, Marcia A.
通讯作者：
Michelin, Marcia A.
影响因子：
5.7
作者：
HAU, DM;CHEN, TH;YOU, JS
通讯作者：
YOU, JS