The dynamics of deltamethrin resistance evolution in Aedes albopictus has an impact on fitness and dengue virus type-2 vectorial capacity.
The dynamics of deltamethrin resistance evolution in Aedes albopictus has an impact on fitness and dengue virus type-2 vectorial capacity.
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DOI：
10.1186/s12915-023-01693-0
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发表时间：
2023-09-13
期刊：
影响因子：
5.4
通讯作者：
Chen, Xiao-Guang
中科院分区：
文献类型：
--
作者：
Guo, Yijia;Hu, Ke;Zhou, Jingni;Xie, Zhensheng;Zhao, Yijie;Zhao, Siyu;Gu, Jinbao;Zhou, Xiaohong;Yan, Guiyun;James, Anthony A.;Chen, Xiao-Guang
白纹伊蚊是登革热、基孔肯雅病和寨卡病毒的重要传播媒介，其在世界范围内的入侵和扩张已成为全球公共卫生的严重问题。化学杀虫剂是目前控制蚊子数量的主要手段。然而，长期和大规模使用杀虫剂选择了蚊子的抗性，伴随着影响适应性的遗传负荷。从实验室来源的溴氰菊酯抗性Ae中分离并鉴定了一些代表不同抗性机制的实验室菌株。白纹伊蚊在以前的工作中恢复。评价并比较了菌株的抗性水平和适合度成本，以表征抗性基因型和表型的演变。电压门控钠通道（vgsc）基因产物（VGSC）中的杂合F1534 S突变（1534 F/S）首先在抗性进化的早期阶段被检测到，不仅赋予高水平的抗性，而且不产生显著的适应性成本，导致抗性在种群中的快速传播。其次是增加频率的纯合子F1534 S（1534 S/S）蚊子，有显着的健身劣势，促使出现一个unlinked I1532 T突变，副作用少，交配优势更好地适应选择和生殖压力施加在实验中。代谢抗性没有显着的健身成本和介导的高耐受性抗性表型可能在随后的抗性进化中发挥主导作用。不同的耐药株对登革2型病毒（DENV-2）具有相似的载体感受态。此外，媒介能力的比较分析表明，由于溴氰菊酯抗性增加的存活平衡了负适应性成本效应，并增加了抗性种群传播登革病毒（DENV）的风险。抗性的逐步进化导致蚊子具有靶位点不敏感性和代谢抗性，具有较低的适应性成本，这进一步导致具有高抗性水平和媒介能力的抗性种群。本研究揭示了白纹伊蚊对溴氰菊酯抗性进化的可能机制。这些发现将有助于指导杀虫剂使用、抗药性管理以及预防和控制蚊媒疾病的实用战略。在线版本包含补充材料，可通过10.1186/s12915-023-01693-0获得。
Worldwide invasion and expansion of Aedes albopictus, an important vector of dengue, chikungunya, and Zika viruses, has become a serious concern in global public health. Chemical insecticides are the primary means currently available to control the mosquito populations. However, long-term and large-scale use of insecticides has selected for resistance in the mosquito that is accompanied by a genetic load that impacts fitness. A number of laboratory strains representing different resistance mechanisms were isolated and identified from laboratory-derived, deltamethrin-resistant Ae. albopictus recovered in previous work. Resistance levels and fitness costs of the strains were evaluated and compared to characterize the evolution of the resistance genotypes and phenotypes. The heterozygous F1534S mutation (1534F/S) in the voltage gated sodium channel (vgsc) gene product (VGSC), first detected in early stages of resistance evolution, not only confers high-level resistance, but also produces no significant fitness costs, leading to the rapid spread of resistance in the population. This is followed by the increase in frequency of homozygous F1534S (1534S/S) mosquitoes that have significant fitness disadvantages, prompting the emergence of an unlinked I1532T mutation with fewer side effects and a mating advantage better adapted to the selection and reproductive pressures imposed in the experiments. Metabolic resistance with no significant fitness cost and mediating a high-tolerance resistance phenotype may play a dominant role in the subsequent evolution of resistance. The different resistant strains had similar vector competence for dengue virus type-2 (DENV-2). Furthermore, a comparative analysis of vectorial capacity revealed that increased survival due to deltamethrin resistance balanced the negative fitness cost effects and contributed to the risk of dengue virus (DENV) transmission by resistant populations. The progressive evolution of resistance results in mosquitoes with both target-site insensitivity and metabolic resistance with lower fitness costs, which further leads to resistant populations with both high resistance levels and vectorial capacity. This study reveals a possible mechanism for the evolution of deltamethrin resistance in Aedes albopictus. These findings will help guide practical strategies for insecticide use, resistance management and the prevention and control of mosquito-borne disease. The online version contains supplementary material available at 10.1186/s12915-023-01693-0.
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影响因子：
3.2
作者：
Diniz DF;de Melo-Santos MA;Santos EM;Beserra EB;Helvecio E;de Carvalho-Leandro D;dos Santos BS;de Menezes Lima VL;Ayres CF
通讯作者：
Ayres CF
疟疾载体动脉藻类动物疟原虫感染和杀虫剂抗性的相互作用。
影响因子：
4.6
作者：
Alout H;Dabiré RK;Djogbénou LS;Abate L;Corbel V;Chandre F;Cohuet A
通讯作者：
Cohuet A
DOI：
10.1017/s001667230100547x
发表时间：
2002-02-01
期刊：
GENETICAL RESEARCH
影响因子：
--
作者：
Berticat, C;Boquien, G;Chevillon, C
通讯作者：
Chevillon, C
DOI：
10.3390/v13112232
发表时间：
2021-11-05
期刊：
Viruses
影响因子：
--
作者：
Stephenson CJ;Coatsworth H;Waits CM;Nazario-Maldonado NM;Mathias DK;Dinglasan RR;Lednicky JA
通讯作者：
Lednicky JA
影响因子：
4.8
作者：
Guo, Yijia;Zhou, Jingni;Chen, Xiao-Guang
通讯作者：
Chen, Xiao-Guang