Self-accelerated electrochemiluminescence emitters of Ag@SnO2 nanoflowers for sensitive detection of cardiac troponin T
Self-accelerated electrochemiluminescence emitters of Ag@SnO2 nanoflowers for sensitive detection of cardiac troponin T
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Ag@SnO2 纳米花的自加速电化学发光发射器用于灵敏检测心肌肌钙蛋白 T
DOI：
10.1016/j.electacta.2018.03.177
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发表时间：
2018-05
影响因子：
6.6
通讯作者：
Zhuo Ying
中科院分区：
文献类型：
--
作者：
Jiang Ming Hui;Lu Pei;Lei Yan Mei;Chai Ya Qin;Yuan Ruo;Zhuo Ying
金属氧化物半导体纳米晶体（NCs）作为一种新型的电化学发光（ECL）发射材料，具有低成本和良好的生物相容性，在ECL生物传感器领域引起了广泛的研究兴趣。然而，它们的分析应用仍然面临着相对较低ECL强度的重大挑战。本文首次在sno2纳米花表面原位生成银纳米粒子，通过银-银镜像反应制备了银纳米粒子官能化sno2纳米花（Ag@SnO2NFs）的自加速ECL发射体，在S2O82−为助反应物的情况下，其ECL发射强度高，最大发射峰在542 nm处。值得注意的是，与直径小于10 nm的SnO2NCs的传统ECL发射器相比，获得的直径约为1-2 μM的Ag@SnO2NFs具有更强的ECL发射。ECL增强的原因可能是：1)超薄sno2纳米片聚集形成的花状纳米结构具有很强的尺寸效应，会产生大量的锡间隙或氧空位；2)合成银纳米粒子（Ag NPs）作为共反应促进剂，产生更多氧化中间体，显著促进Ag@SnO2NFs的ECL排放。考虑到这些特性，我们开发了一种灵敏的ECL免疫传感器，用于检测心脏肌钙蛋白T (cTnT)，利用Ag@SnO2NFs作为有效的信号探针，该传感器具有出色的灵敏度，线性范围为1 fg/mL至100 pg/mL，检测限相对较低，为0.11 fg/mL。这项工作有效地为Ag@SnO2NFs作为低毒性和优越的ECL排放物在ECL生物分析中的有前途的应用提供了见解。
Metal oxide semiconductor nanocrystals (NCs), which emerged as a novel class of electrochemiluminescence (ECL) emitters with low cost and good biocompatibility, have attracted particular research interests in ECL biosensors. However, their analytical applications still remained a substantial challenge of relatively low ECL intensity. Herein, the novel self-accelerated ECL emitters of silver nanoparticles functionalized SnO2nanoflowers (Ag@SnO2NFs) were first prepared byin situgenerating silver nanoparticles on the surface of SnO2nanoflowersviasilver mirror reaction, which exhibited high-intensity ECL emission with the maximum emission peak at 542 nm in the case of S2O82−as a co-reactant. It was worthy to note that compared with traditional ECL emitters of SnO2NCs with diameters below 10 nm, the obtained Ag@SnO2NFs with diameters about 1–2 μM showed a stronger ECL emission. The ECL enhancement was supposed to derive from 1) the strong size effect of flower-like nanostructure aggregating by ultrathin SnO2nanosheets, which would offer large amount of tin interstitials or oxygen vacancies; 2) thein situgenerated silver nanoparticles (Ag NPs) as co-reaction accelerator, which would generate more oxidizing intermediate to significantly self-promote the ECL emission of Ag@SnO2NFs. Given these attributes, we developed a sensitive ECL immunosensor for the detection of cardiac troponin T (cTnT) utilizing Ag@SnO2NFs as efficient signal probes, which showed outstanding sensitivity with a favorable linear range from 1 fg/mL to 100 pg/mL and relatively low detection limit of 0.11 fg/mL. This work efficiently offered insights into the promising applications of Ag@SnO2NFs as a low toxicity and superior ECL emitter in ECL bioanalysis.
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