Biomass production of hairy roots of Artemisia annua and Arachis hypogaea in a scaled-up mist bioreactor.
Biomass production of hairy roots of Artemisia annua and Arachis hypogaea in a scaled-up mist bioreactor.
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DOI：
10.1002/bit.22892
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发表时间：
2010-12-01
影响因子：
3.8
通讯作者：
Weathers, Pamela J.
中科院分区：
文献类型：
--
作者：
Sivakumar, Ganapathy;Liu, Chunzhao;Towler, Melissa J.;Weathers, Pamela J.
毛状根有可能产生各种有价值的小分子和大分子。雾化反应器是一种气相生物反应器，已显示出低成本培养毛状根的前景。用一种较新的一次性培养袋，在1~20L的尺度上对黄花蒿和花生两种植物的毛状根进行了雾化反应器性能的研究，这两种毛状根在雾化反应器中的生长速度都超过了摇瓶培养的毛状根的生长速度。从1 L收集的信息，进一步将花生规模扩大到4 L，然后是20 L。搅拌占空比、培养基流量和增加流量的时间都不同。在雾化反应器中，增加雾化周期或增加介质流速是增加向根床输送液体养分的两种选择。超过2-3分钟的喷雾周期通常被认为不利于生长。另一方面，在20个L反应器中，提高音速喷嘴的介质流量是提高生长速率和生物量的最重要的因素，特别是在根生长的指数期(2-3周)。在1号L反应器中，低矮曲霉的生长量为μ=0.173 d−1，生物量产量为12.75g dWL−1，高于摇瓶中μ=0.166 d−1和11.10g dWL−1时的生长速度和生物量；在20 L反应器中，生长速度和生物量产量最高的分别为μ=0.147和7.77gdWL−1，这主要是在增加中流量输送的情况下实现的。使用这一较新的反应堆设计进一步评估了雾沉积模型，当考虑到根部(+毛发)的表观厚度时，经验数据与模型预测相关联。这些结果为进一步优化雾化生物反应器培养毛状根奠定了基础。
Hairy roots have the potential to produce a variety of valuable small and large molecules. The mist reactor is a gas phase bioreactor that has shown promise for low-cost culture of hairy roots. Using a newer, disposable culture bag, mist reactor performance was studied with two species, Artemisia annua L. and Arachis hypogaea (peanut), at scales from 1 to 20 L. Both species of hairy roots when grown at 1 L in the mist reactor showed growth rates that surpassed that in shake flasks. From the information gleaned at 1 L, Arachis was scaled further to 4 and then 20 L. Misting duty cycle, culture medium flow rate, and timing of when flow rate was increased were varied. In a mist reactor increasing the misting cycle or increasing the medium flow rate are the two alternatives for increased delivery of liquid nutrients to the root bed. Longer misting cycles beyond 2–3 min were generally deemed detrimental to growth. On the other hand, increasing the medium flow rate to the sonic nozzle especially during the exponential phase of root growth (weeks 2–3) was the most important factor for increasing growth rates and biomass yields in the 20 L reactors. A. hypogaea growth in 1 L reactors was μ = 0.173 day−1 with biomass yield of 12.75 g DWL−1. This exceeded that in shake flasks at μ = 0.166 day−1 and 11.10 g DWL−1. Best growth rate and biomass yield at 20 L was μ = 0.147 and 7.77 g DWL−1, which was mainly achieved when medium flow rate delivery was increased. The mist deposition model was further evaluated using this newer reactor design and when the apparent thickness of roots (+hairs) was taken into account, the empirical data correlated with model predictions. Together these results establish the most important conditions to explore for future optimization of the mist bioreactor for culture of hairy roots.
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影响因子：
3.8
作者：
Kim, YJ;Weathers, PJ;Wyslouzil, BE
通讯作者：
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影响因子：
3.8
作者：
Kim, YJ;Weathers, PJ;Wyslouzil, BE
通讯作者：
Wyslouzil, BE