Population genetic analysis and sub-structuring of Theileria parva in the northern and eastern parts of Zambia.
Population genetic analysis and sub-structuring of Theileria parva in the northern and eastern parts of Zambia.
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DOI：
10.1186/1756-3305-5-255
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发表时间：
2012-11-12
影响因子：
3.2
通讯作者：
Sawa H
中科院分区：
文献类型：
--
作者：
Muleya W;Namangala B;Simuunza M;Nakao R;Inoue N;Kimura T;Ito K;Sugimoto C;Sawa H
由细小芽孢杆菌引起的芽孢杆菌病在非洲是一种重要的经济疾病。这是制约非洲东部、中部和南部一些地区畜牧业发展的主要因素。在赞比亚，他们的菌痢病每年造成多达1万头牛的损失。收集了来自赞比亚Isoka和petake地区的牛血液样本，用于对细小芽孢杆菌进行遗传分析。然后使用9个微卫星标记对所有细小芽孢杆菌阳性样品进行PCR分析。利用微卫星工具包GenAlEx ver对微卫星数据进行分析。开始吧。2.9.3.2、LIAN计算机软件。p104基因引物PCR检测两区总阳性检出率为54.9% (95% CI: 46.7 ~ 63.1%, 78/142)， Isoka区为44.8% (95% CI: 34.8 ~ 54.8%)， petake区为76.1% （95% CI = 63.9 ~ 88.4%）。利用包含4条染色体的9个微卫星标记，对61份样品（Isoka 33份，petaauke 28份）的细小芽孢杆菌群体遗传结构进行了分析。Wright’s F指数（FST = 0.178）表明Isoka和petake种群之间存在显著差异。当两个地区的人口被视为单一人口时，观察到连锁不平衡。当单独分析时，在Isoka区的Kanyelele和Kalembe地区，Isoka区的整体和petake区观察到连锁不平衡。Petauke区感染的多重性高于Isoka区。Isoka和petake地区的小孢子细孢菌群体遗传分析表明，地区间基因型交换水平较低，但各地区群体内遗传多样性较高，表明地区间存在遗传亚结构和地理亚结构。观察到的亚结构以及种群中缺乏泛菌病，可能是由于采样时的低传播水平所致。然而，伊索卡种群的多样性低于佩托克种群。
Theileriosis, caused by Theileria parva, is an economically important disease in Africa. It is a major constraint to the development of the livestock industry in some parts of eastern, central and southern Africa. In Zambia, theileriosis causes losses of up to 10,000 cattle annually. Cattle blood samples were collected for genetic analysis of Theileria parva from Isoka and Petauke districts in Zambia. Microsatellite analysis was then performed on all Theileria parva positive samples for PCR using a panel of 9 microsatellite markers. Microsatellite data was analyzed using microsatellite toolkit, GenAlEx ver. 6, Fstat ver. 2.9.3.2, and LIAN computer softwares. The combined percentage of positive samples in both districts determined by PCR using the p104 gene primers was 54.9% (95% CI: 46.7 – 63.1%, 78/142), while in each district, it was 44.8% (95% CI: 34.8 – 54.8%) and 76.1% (95% CI = 63.9 – 88.4%) for Isoka and Petauke districts, respectively. We analyzed the population genetic structure of Theileria parva from a total of 61 samples (33 from Isoka and 28 from Petauke) using a panel of 9 microsatellite markers encompassing the 4 chromosomes of Theileria parva. Wright’s F index (FST = 0.178) showed significant differentiation between the Isoka and Petauke populations. Linkage disequilibrium was observed when populations from both districts were treated as a single population. When analyzed separately, linkage disequilibrium was observed in Kanyelele and Kalembe areas in Isoka district, Isoka district overall and in Petauke district. Petauke district had a higher multiplicity of infection than Isoka district. Population genetic analyses of Theileria parva from Isoka and Petauke districts showed a low level of genotype exchange between the districts, but a high level of genetic diversity within each district population, implying genetic and geographic sub-structuring between the districts. The sub-structuring observed, along with the lack of panmixia in the populations, could have been due to low transmission levels at the time of sampling. However, the Isoka population was less diverse than the Petauke population.
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影响因子：
64.8
作者：
BOWCOCK, AM;RUIZLINARES, A;CAVALLISFORZA, LL
通讯作者：
CAVALLISFORZA, LL
影响因子：
3.2
作者：
Ouma, Johnson O.;Beadell, Jon S.;Caccone, Adalgisa
通讯作者：
Caccone, Adalgisa
影响因子：
2
作者：
GAUER, M;MACKENSTEDT, U;MORZARIA, S
通讯作者：
MORZARIA, S
影响因子：
1.5
作者：
Simuunza, Martin;Bilgic, Huseyin;Weir, William
通讯作者：
Weir, William
影响因子：
3.2
作者：
Tanekou, Tresor Tito;Melachio, T. T.;Njiokou, Flobert
通讯作者：
Njiokou, Flobert