Comparison of Surface Persistence of SARS-CoV-2 Alpha and Delta Variants on Stainless Steel at 4°C and 24°C.
Comparison of Surface Persistence of SARS-CoV-2 Alpha and Delta Variants on Stainless Steel at 4°C and 24°C.
复制标题
SARS-COV-2α和三角洲变体在4°C和24°C下的表面持久性比较。
DOI：
10.1128/aem.00764-22
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发表时间：
2022-07-26
影响因子：
4.4
通讯作者：
Bennett, Allan M.
中科院分区：
文献类型：
--
作者：
Onianwa, Okechukwu;Garratt, Isobel;Carter, Jennifer;Spencer, Antony;Verlander, Neville Q.;Pottage, Thomas;Bennett, Allan M.
关键词：
大多数关于SARS-CoV-2表面持久性的研究都是在20°C到30°C之间进行的，关于SARS-CoV-2在低温下存活的数据有限。在这项研究中，研究了SARS-CoV-2Alpha和Delta变异株在两种温度(4℃和24℃)下在不锈钢上的稳定性。结果表明，与4℃相比，两种变异体在24℃时的衰减速度都更快，在24℃时，Alpha变种和Delta变种在前2.5 h内的衰减率分别为0.33log10和1.02log10，而在4℃时，Alpha变异体在前2.5 h内的衰减率为0.16log10，而Delta变异体没有观察到减少。在24℃下原位放置24小时后，观察到log10减少了2.66(Alpha)和3.11(Delta)。分别在48 h和72 h后未发现有活性的Alpha和Delta变异体。在冷藏环境中(4℃)24小时后，观察到对数10减少了1.16(Alpha)和0.95(Delta)。在本实验条件下，两种病毒在不锈钢上至少存活了1 周。在10 天后，没有发现有活性的阿尔法和德尔塔变种。这些发现支持在全球较冷地区和一些工业部门冬季几个月内SARS-CoV-2混合传播增加的可能性。重要信息人类传播据信主要通过直接传播传染性飞沫或气雾剂。然而，通过接触受污染的表面传播甲藻也可能起到重要作用。这项研究提供了新的证据，比较了阿尔法和德尔塔变种在4°C和24°C下在不锈钢表面的稳定性，发现这两种变种在4°C下仍可检测到长达7 天。在24℃下，Delta型和Alpha型相比，Delta型和Alpha型在2 d内均可恢复，而Alpha型在2 d后不能恢复。这对在寒冷环境中生活和工作的人进行福美拉特传播干预具有重要意义。
Most studies on surface persistence of SARS-CoV-2 have been conducted at temperatures between 20°C and 30°C. There is limited data on the survival of SARS-CoV-2 at low temperatures. In this study, the stability of SARS-CoV-2 Alpha and Delta variants on stainless steel was investigated at two temperatures (4°C and 24°C). The results show that both variants decayed more rapidly at 24°C compared with 4°C. At 24°C, Alpha and Delta variants showed reductions of 0.33 log10 and 1.02 log10, respectively, within the first 2.5 h. However, at 4°C, Alpha variant showed a reduction of 0.16 log10 within the first 2.5 h while no reduction was observed with Delta variant. After remaining in situ for 24 h at 24°C, log10 reductions of 2.66 (Alpha) and 3.11 (Delta) were observed. No viable Alpha and Delta variant was recovered after 48 h and 72 h, respectively. After 24 h in a refrigerated environment (4°C) log10 reductions of 1.16 (Alpha) and 0.95 (Delta) were observed. Under these experimental conditions, both viruses survived on stainless steel for at least 1 week. No viable Alpha and Delta variant was recovered after 10 days. These findings support the potential for increased fomite transmission of SARS-CoV-2 during winter months in colder regions worldwide and in some industrial sectors. IMPORTANCE Human transmission is believed to occur primarily through direct transfer of infectious droplets or aerosols. However, fomite transmission through contact with contaminated surfaces may also play an important role. This study provides novel evidence comparing the stability of Alpha and Delta variants on stainless steel surfaces at 4°C and 24°C. At 4°C both variants were found to be still detectable for up to 7 days. At 24°C Delta variant could be recovered over 2 days compared with Alpha variant which could not be recovered after 2 days. This has implications for fomite transmission interventions for people living and working in cold environments.
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