Signatures of Insecticide Selection in the Genome of Drosophila melanogaster.
Signatures of Insecticide Selection in the Genome of Drosophila melanogaster.
复制标题
在果蝇果蝇基因组中选择杀虫剂的特征。
DOI：
10.1534/g3.118.200537
复制
发表时间：
2018-11-06
期刊：
影响因子：
--
通讯作者：
Buchon N
中科院分区：
文献类型：
--
作者：
Duneau D;Sun H;Revah J;San Miguel K;Kunerth HD;Caldas IV;Messer PW;Scott JG;Buchon N
对杀虫剂的抗药性已经在多种昆虫物种中进化，导致施用率增加，甚至控制失败。了解杀虫剂抗性的遗传基础对于减轻其对作物生产和疾病控制的影响至关重要。我们使用果蝇遗传参考小组（DGRP）进行了GWAS方法，以确定对两种广泛使用的杀虫剂：有机磷酸酯（OP，敌百虫）和拟除虫菊酯（溴氰菊酯）的抗性所涉及的突变。大多数抗性变异与靶基因Ace突变相关，而大多数溴氰菊酯抗性变异与Cyp6a23突变相关，Cyp6a23是一种编码解毒酶的基因，以前从未与抗性相关。“巢式GWAS”进一步揭示了其他基因座的贡献：Dscam 1和trpl与抗草甘膦有关，但仅在缺乏沃尔巴克氏体的品系中。Cyp6a23的旁系同源基因Cyp6a17和ATP结合盒转运蛋白CG7627与溴氰菊酯抗性有关。我们在所有这些耐药基因座观察到最近选择性扫描的特征，并证实Ace的软扫描确实是由鉴定的耐药突变驱动的。对其他人群样本中等位基因频率的分析显示，大多数耐药突变在地球仪上分离，但不同人群之间的频率差异很大。总之，我们的数据表明，广泛使用的OP和拟除虫菊酯杀虫剂对自然昆虫种群施加了强大的选择压力。然而，目前尚不清楚为什么在果蝇中，抗性的进化是由于OP靶位点的变化，而是由于拟除虫菊酯的解毒酶。
Resistance to insecticides has evolved in multiple insect species, leading to increased application rates and even control failures. Understanding the genetic basis of insecticide resistance is fundamental for mitigating its impact on crop production and disease control. We performed a GWAS approach with the Drosophila Genetic Reference Panel (DGRP) to identify the mutations involved in resistance to two widely used classes of insecticides: organophosphates (OPs, parathion) and pyrethroids (deltamethrin). Most variation in parathion resistance was associated with mutations in the target gene Ace, while most variation in deltamethrin resistance was associated with mutations in Cyp6a23, a gene encoding a detoxification enzyme never previously associated with resistance. A “nested GWAS” further revealed the contribution of other loci: Dscam1 and trpl were implicated in resistance to parathion, but only in lines lacking Wolbachia. Cyp6a17, the paralogous gene of Cyp6a23, and CG7627, an ATP-binding cassette transporter, were implicated in deltamethrin resistance. We observed signatures of recent selective sweeps at all of these resistance loci and confirmed that the soft sweep at Ace is indeed driven by the identified resistance mutations. Analysis of allele frequencies in additional population samples revealed that most resistance mutations are segregating across the globe, but that frequencies can vary substantially among populations. Altogether, our data reveal that the widely used OP and pyrethroid insecticides imposed a strong selection pressure on natural insect populations. However, it remains unclear why, in Drosophila, resistance evolved due to changes in the target site for OPs, but due to a detoxification enzyme for pyrethroids.
登录
查看更多内容
影响因子：
3.4
作者：
Menozzi P;Shi MA;Lougarre A;Tang ZH;Fournier D
通讯作者：
Fournier D
影响因子：
4.6
作者：
Denecke S;Fusetto R;Martelli F;Giang A;Battlay P;Fournier-Level A;O' Hair RA;Batterham P
通讯作者：
Batterham P
影响因子：
5.1
作者：
FOURNIER, D;MUTERO, A;BRIDE, JM
通讯作者：
BRIDE, JM
DOI：
10.1534/g3.116.031054
发表时间：
2016-08-09
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Battlay P;Schmidt JM;Fournier-Level A;Robin C
通讯作者：
Robin C
影响因子：
16.8
作者：
Messer PW;Petrov DA
通讯作者：
Petrov DA