Over-Expression of the Cell-Cycle Gene LaCDKB1;2 Promotes Cell Proliferation and the Formation of Normal Cotyledonary Embryos during Larix kaempferi Somatic Embryogenesis.
Over-Expression of the Cell-Cycle Gene LaCDKB1;2 Promotes Cell Proliferation and the Formation of Normal Cotyledonary Embryos during Larix kaempferi Somatic Embryogenesis.
复制标题
细胞周期基因LaCDKB1;2过表达促进落叶松体细胞胚胎发生过程中细胞增殖和正常子叶胚胎的形成
作者：
Kang Y;Li W;Zhang L;Qi L
体细胞胚胎发生是培育具有理想特性的林木幼苗的有效手段，但高质量成熟体细胞胚胎的萌发率和繁殖力仍然是日本落叶松的限制因素。在此，我们分析了日本落叶松在体细胞胚胎发生过程中细胞周期蛋白依赖性激酶B1；2(LaCDKB1；2)的表达模式及其与细胞增殖率的关系。我们还分析了LaCDKB1；2过表达对体细胞胚胎质量的影响。结果表明，在增殖期，LaCDKB1；2的表达与细胞增殖率呈正相关。LaCDKB1；2过表达后，细胞增殖率提高，转基因细胞的体细胞胚胎数是未转化细胞的2.69倍。值得注意的是，在转基因培养中，正常子叶胚胎的数量是未转化培养中的3倍，这表明LaCDKB1；2不仅增加了培养物的增殖和体细胞胚胎的数量，而且还提高了体细胞胚胎的质量。这些结果为深入研究落叶松体细胞胚胎发生的调控机制以及寻找新的育种材料提供了依据。
Somatic embryogenesis is an effective tool for the production of forest tree seedlings with desirable characteristics; however, the low initiation frequency and productivity of high-quality mature somatic embryos are still limiting factors for Larix kaempferi (Japanese larch). Here, we analyzed the expression pattern of L. kaempferi cyclin-dependent kinase B 1;2 (LaCDKB1;2) during somatic embryogenesis in L. kaempferi and its relationship with the cell proliferation rate. We also analyzed the effect of LaCDKB1;2 over-expression on somatic embryo quality. The results revealed a positive correlation between LaCDKB1;2 expression and the cell proliferation rate during the proliferation stage. After LaCDKB1;2 over-expression, the proliferation rate of cultures increased, and the number of somatic embryos in transgenic cultures was 2.69 times that in non-transformed cultures. Notably, the number of normal cotyledonary embryos in transgenic cultures was 3 times that in non-transformed cultures, indicating that LaCDKB1;2 not only increases the proliferation of cultures and the number of somatic embryos but also improves the quality of somatic embryos. These results provide insight into the regulatory mechanisms of somatic embryogenesis as well as new Larix breeding material.
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影响因子：
6.9
作者：
Czerednik A;Busscher M;Bielen BA;Wolters-Arts M;de Maagd RA;Angenent GC
通讯作者：
Angenent GC
影响因子：
0.7
作者：
Ander, Castander-Olarieta;Paloma, Moncalean;Itziar, Montalban A.
通讯作者：
Itziar, Montalban A.
影响因子：
2.9
作者：
Fan, Yanru;Li, Zhexin;Qi, Liwang
通讯作者：
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影响因子：
11.6
作者：
LIU, ZB;ULMASOV, T;GUILFOYLE, TJ
通讯作者：
GUILFOYLE, TJ
氢处理落叶松体细胞胚胎 mRNA 和 miRNA 转录组分析
影响因子：
5.6
作者：
Liu Y;Han S;Ding X;Li X;Zhang L;Li W;Xu H;Li Z;Qi L
通讯作者：
Qi L