Microcurrent stimulation activates the circadian machinery in mice.
Microcurrent stimulation activates the circadian machinery in mice.
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微电流刺激激活小鼠的昼夜节律机制。
DOI：
10.1016/j.bbrc.2019.02.022
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Ohdo S.
中科院分区：
文献类型：
--
作者：
Matsunaga N;Yoshida Y;Kitajou N;Shiraishi A;Kusunose N;Koyanagi S;Ohdo S.
生物钟基因簇在转录水平上控制着生物钟的昼夜节律，调节着人体的各种功能。生物钟基因与各种疾病的病理有关，而这些疾病又与衰老有关。人类的衰老是一个世界性的问题;它会导致睡眠障碍和昼夜节律的破坏。它还降低了眼睛的视力和食欲，削弱了光和食物对生物钟基因的同步作用。因此，需要一种简单的方法来同步体内的时钟基因。本研究探讨了微电流刺激（MCS）对野生型（WT）和Clk/Clk小鼠昼夜节律机制的影响。MCS诱导培养的小鼠星形胶质细胞Per 1 mRNA表达，Per 1启动子中的cAMP反应元件（CRE）对Per 1 mRNA的诱导起重要作用。此外，MCS还可增加小鼠肝脏Per 1 mRNA的表达，并使Per 1表达节律的时相提前。磷酸化cAMP反应元件结合蛋白（pCREB）表达节律改变，表达时相提前。最后，研究了MCS对WT和Clk/Clk小鼠自发活动节律的影响。MCS可使WT和Clk/Clk小鼠自发活动节律的时相提前。这项研究的结果表明，MCS激活了小鼠的生物钟机制;因此，MCS可能会提高未来新治疗方式的质量。
The circadian rhythm, which regulates various body functions, is transcriptionally controlled by a series of clock gene clusters. The clock genes are related to the pathology of various kinds of diseases, which in turn, is related to aging. Aging in humans is a worldwide problem; it induces sleep disorders and disruption of the circadian rhythm. It also decreases ocular vision and appetite and weakens the synchronization of clock genes by light and food. Therefore, a simple method for the synchronization of clock genes in the body is required. In this study, the influence of microcurrent stimulation (MCS) on the circadian machinery in wild-type (WT) andClockmutant (Clk/Clk) mice was investigated. MCS inducedPer1mRNA expression in cultured mouse astrocytes; cAMP response element (CRE) in thePer1mouse promoter was found to be important for the induction ofPer1mRNA. In addition, MCS increased thePer1mRNA levels in mouse livers and caused the phase advance of thePer1expression rhythm. The protein expression rhythm of phosphor-cAMP response element-binding protein (pCREB) was altered and the phase of expression of pCREB protein advanced. Finally, the influence of MCS on the locomotor activity rhythm in WT andClk/Clkmice was investigated. MCS caused the phase advance of the locomotor activity rhythm in WT andClk/Clkmice. The results of this study indicate that MCS activated the clock machinery in mice; MCS may thus improve the quality of new treatment modalities in the future.
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DOI：
10.1136/bjo.2008.141747
发表时间：
2008-11
期刊：
The British journal of ophthalmology
影响因子：
--
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影响因子：
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影响因子：
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作者：
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