Gene expression profiles responses to aphid feeding in chrysanthemum (Chrysanthemum morifolium).
Gene expression profiles responses to aphid feeding in chrysanthemum (Chrysanthemum morifolium).
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DOI：
10.1186/1471-2164-15-1050
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发表时间：
2014-12-02
期刊：
影响因子：
4.4
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Xia X;Shao Y;Jiang J;Ren L;Chen F;Fang W;Guan Z;Chen S
菊花是世界各地重要的观赏植物。它很容易受到蚜虫Macrosiphoniella sanbourni的攻击。植物对蚜虫防御反应的分子机制目前还不完全清楚。本文利用RNA-Seq技术研究了菊花叶片中蚜虫取食后基因表达的变化。从在有（Y）或没有（CK）蚜虫侵染和模拟穿刺处理（Z）的不同时间点收集的菊花‘nannongxunzhang’的汇集的叶组织产生三个文库，并使用Illumina HiSeqTM 2000平台测序。在文库CK、Y和Z中分别获得总共7，363，292、7，215，860和7，319，841个干净读段。每个文库中干净读段的比例>97.29%。将大约76.35%的干净读段映射到包括所有已知的菊花单基因序列的参考基因数据库。CK-VS-Y、CK-VS-Z和Z-VS-Y的差异表达基因（DEG）分别为1,157、527和340个。这些DEG参与植物激素信号转导、细胞壁生物合成、光合作用、活性氧（ROS）途径和转录因子调控网络等，蚜虫取食诱导的基因表达变化是多方面的。这些基因所属的不同途径之间存在各种形式的串扰，这将使植物能够微调其防御反应。本文的在线版本（doi：10.1186/1471-2164-15-1050）包含补充材料，可供授权用户使用。
Chrysanthemum is an important ornamental plant all over the world. It is easily attacked by aphid, Macrosiphoniella sanbourni. The molecular mechanisms of plant defense responses to aphid are only partially understood. Here, we investigate the gene expression changes in response to aphid feeding in chrysanthemum leaf by RNA-Seq technology. Three libraries were generated from pooled leaf tissues of Chrysanthemum morifolium ‘nannongxunzhang’ that were collected at different time points with (Y) or without (CK) aphid infestations and mock puncture treatment (Z), and sequenced using an Illumina HiSeqTM 2000 platform. A total of 7,363,292, 7,215,860 and 7,319,841 clean reads were obtained in library CK, Y and Z, respectively. The proportion of clean reads was >97.29% in each library. Approximately 76.35% of the clean reads were mapped to a reference gene database including all known chrysanthemum unigene sequences. 1,157, 527 and 340 differentially expressed genes (DEGs) were identified in the comparison of CK-VS-Y, CK-VS-Z and Z-VS-Y, respectively. These DEGs were involved in phytohormone signaling, cell wall biosynthesis, photosynthesis, reactive oxygen species (ROS) pathway and transcription factor regulatory networks, and so on. Changes in gene expression induced by aphid feeding are shown to be multifaceted. There are various forms of crosstalk between different pathways those genes belonging to, which would allow plants to fine-tune its defense responses. The online version of this article (doi:10.1186/1471-2164-15-1050) contains supplementary material, which is available to authorized users.
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