Evidence of recent interkingdom horizontal gene transfer between bacteria and Candida parapsilosis.
Evidence of recent interkingdom horizontal gene transfer between bacteria and Candida parapsilosis.
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DOI：
10.1186/1471-2148-8-181
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发表时间：
2008-06-24
影响因子：
3.4
通讯作者：
Butler, Geraldine
中科院分区：
文献类型：
--
作者：
Fitzpatrick, David A.;Logue, Mary E.;Butler, Geraldine
到目前为止，很少有域间基因转移到真菌中的事件被确定。在这里，我们使用白色念珠菌WO-1、热带念珠菌、近端念珠菌、卢西坦克氏杆菌、吉列蒙氏毕赤酵母和长孢杆菌的新基因组序列来鉴定最近发生的跨域HGT事件。我们将这些物种称为CTG物种，因为它们将CTG密码子翻译为丝氨酸而不是亮氨酸，并分享了最近的共同祖先。系统发育和同线信息推测，两个近缘种弧菌基因起源于细菌。一种编码一个可能的脯氨酸消旋酶(PR)。系统发育分析还推断，细菌PR酶有独立的转移到芽孢杆菌和原生生物的成员中。副隐孢子虫的第二个HGT基因属于吩嗪F(PhzF)超家族。大多数CTG物种还含有真菌PhzF同源物。我们的系统发育表明，CTG同源基因起源于一个古老的HGT事件，来自蛋白细菌的一个成员。Synteny的分析表明，近平弧菌已经失去了内源真菌形式的PhzF，随后又从一种蛋白细菌来源重新获得了它。有证据表明，裂殖酵母和担子酵母也通过HGT获得了PhzF同源物。我们的搜索发现了两个例子，从细菌到CTG分支的HGT得到了很好的支持，都是近缘念珠菌所特有的。因此，虽然最近的跨王国基因转移发生在CTG谱系中，但它的发生是罕见的。然而，我们的分析不会检测到古老的基因转移，而且我们可能低估了HGT进入CTG物种的全球范围。
To date very few incidences of interdomain gene transfer into fungi have been identified. Here, we used the emerging genome sequences of Candida albicans WO-1, Candida tropicalis, Candida parapsilosis, Clavispora lusitaniae, Pichia guilliermondii, and Lodderomyces elongisporus to identify recent interdomain HGT events. We refer to these as CTG species because they translate the CTG codon as serine rather than leucine, and share a recent common ancestor. Phylogenetic and syntenic information infer that two C. parapsilosis genes originate from bacterial sources. One encodes a putative proline racemase (PR). Phylogenetic analysis also infers that there were independent transfers of bacterial PR enzymes into members of the Pezizomycotina, and protists. The second HGT gene in C. parapsilosis belongs to the phenazine F (PhzF) superfamily. Most CTG species also contain a fungal PhzF homolog. Our phylogeny suggests that the CTG homolog originated from an ancient HGT event, from a member of the proteobacteria. An analysis of synteny suggests that C. parapsilosis has lost the endogenous fungal form of PhzF, and subsequently reacquired it from a proteobacterial source. There is evidence that Schizosaccharomyces pombe and Basidiomycotina also obtained a PhzF homolog through HGT. Our search revealed two instances of well-supported HGT from bacteria into the CTG clade, both specific to C. parapsilosis. Therefore, while recent interkingdom gene transfer has taken place in the CTG lineage, its occurrence is rare. However, our analysis will not detect ancient gene transfers, and we may have underestimated the global extent of HGT into CTG species.
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影响因子：
64.8
作者：
Dujon, B;Sherman, D;Souciet, JL
通讯作者：
Souciet, JL
影响因子：
3.1
作者：
Brinkman, FSL;MacFarlane, ELA;Hancock, REW
通讯作者：
Hancock, REW
影响因子：
30.8
作者：
Friesen, Timothy L.;Stukenbrock, Eva H.;Oliver, Richard P.
通讯作者：
Oliver, Richard P.
影响因子：
14.9
作者：
Bullerwell, CE;Leigh, J;Lang, BF
通讯作者：
Lang, BF
影响因子：
--
作者：
Hall, C;Brachat, S;Dietrich, FS
通讯作者：
Dietrich, FS