A novel chlorhexidine-hexametaphosphate coating for titanium with antibiofilm efficacy and stem cell cytocompatibility.
A novel chlorhexidine-hexametaphosphate coating for titanium with antibiofilm efficacy and stem cell cytocompatibility.
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DOI：
10.1007/s10856-021-06616-5
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发表时间：
2021-11-20
期刊：
影响因子：
--
通讯作者：
Barbour ME
中科院分区：
文献类型：
--
作者：
Garner SJ;Dalby MJ;Nobbs AH;Barbour ME
种植牙是一种越来越流行的替代缺失牙齿的方法。虽然种植体存活率很高，但少数种植体在植入后很快就失败了，原因有多种因素，包括细菌污染，可能会破坏骨整合。这项工作描述了钛合金氯己定-六偏磷酸盐涂层的开发，该涂层可水解释放防腐剂氯己定。目的是开发一种钛涂层，可释放足够的氯己定以防止生物膜形成，同时保持与骨整合相关细胞的细胞相容性。对涂层的物理性质进行了表征，然后使用多物种生物膜模型研究了抗生物膜功效，并使用人间充质干细胞测定了细胞相容性。该涂层在临床使用中表现出与某些植入物表面相似的物理化学特性，并且在长达 72 小时内显着减少多物种生物膜生物量的形成。一种涂层具有优异的细胞相容性，间充质干细胞能够执行正常功能并开始成骨细胞分化，尽管其生长速度比在未涂层钛上生长的速度慢。经过进一步改进，这些涂层可用于预防手术时牙种植体的细菌污染。这可能有助于降低早期种植失败率。
Dental implants are an increasingly popular way to replace missing teeth. Whilst implant survival rates are high, a small number fail soon after placement, with various factors, including bacterial contamination, capable of disrupting osseointegration. This work describes the development of chlorhexidine-hexametaphosphate coatings for titanium that hydrolyse to release the antiseptic agent chlorhexidine. The aim was to develop a coating for titanium that released sufficient chlorhexidine to prevent biofilm formation, whilst simultaneously maintaining cytocompatibility with cells involved in osseointegration. The coatings were characterised with respect to physical properties, after which antibiofilm efficacy was investigated using a multispecies biofilm model, and cytocompatibility determined using human mesenchymal stem cells. The coatings exhibited similar physicochemical properties to some implant surfaces in clinical use, and significantly reduced formation of multispecies biofilm biomass up to 72 h. One coating had superior cytocompatibility, with mesenchymal stem cells able to perform normal functions and commence osteoblastic differentiation, although at a slower rate than those grown on uncoated titanium. With further refinement, these coatings may have application in the prevention of bacterial contamination of dental implants at the time of surgery. This could aid a reduction in rates of early implant failure.
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影响因子：
3.2
作者：
Giannelli, M.;Chellini, F.;Tani, A.
通讯作者：
Tani, A.
DOI：
10.1590/1516-1439.346814
发表时间：
2015-08-01
期刊：
Materials Research
影响因子：
--
作者：
Dal’Agnol, Claudio Zeferino;Stefenon, Letícia;Santos, Luciana Ruschel dos
通讯作者：
Santos, Luciana Ruschel dos
影响因子：
8
作者：
Barbour, Michele E;Maddocks, Sarah E;Collins, Andrew M
通讯作者：
Collins, Andrew M
影响因子：
5.5
作者：
Barbour, Michele E.;Maddocks, Sarah E.;Saunders, Margaret
通讯作者：
Saunders, Margaret
影响因子：
7.6
作者：
Buser, D;Broggini, N;Steinemann, SG
通讯作者：
Steinemann, SG