The interactions of astrocytes and fibroblasts with defined pore structures in static and perfusion cultures.
The interactions of astrocytes and fibroblasts with defined pore structures in static and perfusion cultures.
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DOI：
10.1016/j.biomaterials.2010.11.046
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发表时间：
2011-03
期刊：
影响因子：
14
通讯作者：
Riehle, Mathis O.
中科院分区：
文献类型：
--
作者：
Sun, Tao;Donoghue, Peter S.;Higginson, Jennifer R.;Gadegaard, Nikolaj;Barnett, Susan C.;Riehle, Mathis O.
打开孔隙以维持细胞定植后的营养扩散和废物清除对于成功应用基于组装膜的构建体至关重要，在我们的例子中，管状支架由聚己内酯（PCL）制成，用于组织工程。由于可移植组织所需的这种支架的复杂三维结构和大尺寸，很难原位研究细胞-孔相互作用。因此，开发了含有多孔PCL或聚二甲基硅氧烷（PDMS）膜的皮氏培养皿（直径30 mm）内的小型化生物反应器，以允许在静态和灌注培养期间原位研究具有限定孔的不同细胞的相互作用。对两种不同细胞类型（成纤维细胞和皮质星形胶质细胞）以及它们如何与一系列孔（直径100-350 μm）相互作用长达50天的研究表明，细胞要么“覆盖”要么“桥接"孔。在皮质星形胶质细胞与孔相互作用的方式中观察到三种不同的行为，而成纤维细胞能够基于一致的“共同努力”快速桥接孔。我们的研究表明，这两种细胞类型的不同的孔密封行为的影响，孔径，初始细胞密度和培养时间，但不受介质灌注范围内的剪切力的调查。这些发现形成了关于支架内孔的可用性的重要基础数据，这些数据可以为组织工程中各种应用的合适支架的设计和制造提供信息。
Open pores to maintain nutrient diffusion and waste removal after cell colonization are crucial for the successful application of constructs based on assembled membranes, in our case tubular scaffolds made of ɛ-polycaprolactone (PCL), for use in tissue engineering. Due to the complex three-dimensional structure and large size of such scaffolds needed for transplantable tissues, it is difficult to investigate the cell–pore interactions in situ. Therefore miniaturized bioreactors inside Petri dishes (30 mm in diameter), containing porous PCL or poly-dimethylsiloxane (PDMS) membranes, were developed to allow the interactions of different cells with defined pores to be investigated in situ during both static and perfusion cultures. Investigation of two different cell types (fibroblasts and cortical astrocytes) and how they interact with a range of pores (100–350 μm in diameter) for up to 50 days indicated that the cells either ‘covered’ or ‘bridged’ the pores. Three distinct behaviors were observed in the way cortical astrocytes interacted with pores, while fibroblasts were able to quickly bridge the pores based on consistent “joint efforts”. Our studies demonstrate that the distinct pore sealing behaviors of both cell types were influenced by pore size, initial cell density and culture period, but not by medium perfusion within the range of shear forces investigated. These findings form important basic data about the usability of pores within scaffolds that could inform the design and fabrication of suitable scaffolds for various applications in tissue engineering.
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影响因子：
14
作者：
Mata, Alvaro;Kim, Eun Jung;Boehm, Cynthia A.;Fleischman, Aaron J.;Muschler, George F.;Roy, Shuvo
通讯作者：
Roy, Shuvo
影响因子：
11.4
作者：
NOBLE, M;MURRAY, K
通讯作者：
MURRAY, K
影响因子：
2.3
作者：
Gadegaard, N;Thoms, S;Wilkinson, CDW
通讯作者：
Wilkinson, CDW
影响因子：
13.3
作者：
Csaderova, Lucia;Martines, Elena;Riehle, Mathis O.
通讯作者：
Riehle, Mathis O.
影响因子：
3.8
作者：
Sun, Tao;Norton, David;MacNeil, Sheila
通讯作者：
MacNeil, Sheila