An intratracheal challenge murine model of asthma: can bronchial inflammation affect the nose?
An intratracheal challenge murine model of asthma: can bronchial inflammation affect the nose?
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气管内激发哮喘小鼠模型：支气管炎症会影响鼻子吗？
DOI：
10.4168/aair.2015.7.1.76
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发表时间：
2015-01
期刊：
影响因子：
--
通讯作者：
Zhong N
中科院分区：
文献类型：
--
作者：
Xie J;Xi Y;Zhang Q;Chen G;Wei L;Lai K;Zhong N
大量数据支持变态反应性疾病中上呼吸道对下呼吸道炎症和病理生理的影响。然而，很少有研究关注孤立的下气道过敏原攻击后鼻子的过敏性炎症，这种情况在临床上可能存在，当人类受试者主要通过口腔呼吸时，如鼻塞时发生。本研究使用小鼠模型来研究孤立的下气道过敏原攻击是否会诱导上呼吸道炎症和高反应性。BALB/c小鼠全身腹腔注射卵清蛋白/生理盐水致敏，气管内注射卵清蛋白/生理盐水致敏。通过鼻腔组织和支气管肺泡灌洗液（BALF）的细胞学和组织学来评估鼻和肺的炎症，同时在刺激后3天内测量鼻气道阻力和反应。麻醉小鼠气管内应用过敏原导致下气道排他性沉积。与对照动物相比，卵清蛋白致敏小鼠攻毒后支气管高反应性，血清BALF中IL-5升高，肺部嗜酸性粒细胞浸润。然而，卵清蛋白致敏小鼠的鼻腔组织学显示嗜酸性粒细胞浸润未增加。攻毒后鼻灌洗液中嗜酸性粒细胞和IL-5均未增加，鼻气道阻力也未增加。在小鼠过敏模型中，下气道的排他性过敏原攻击可引起肺部和全身过敏反应，但不会引起上呼吸道炎症或生理反应。
Extensive data support the influence of the upper airway on lower airway inflammation and pathophysiology in allergic disease. However, few studies have focused on allergic inflammation in the nose after an isolated lower airway allergen challenge, a situation that can exist clinically when human subjects breathe primarily through the mouth, as occurs when nasally congested. This study used a mouse model to investigate whether upper airway inflammation and hyperresponsiveness were induced by an isolated lower airway allergen challenge. BALB/c mice were sensitized by systemic intraperitoneal injection of ovalbumin/saline and challenged with intratracheal ovalbumin/saline. Inflammation in the nose and lungs was assessed by cytology and histology of nasal tissues and bronchoalveolar lavage fluid (BALF), while nasal airway resistance and response were measured over 3 days post-challenge. Intratracheal application of an allergen in anaesthetized mice resulted in exclusive deposition in the lower airway. Compared to control animals, ovalbumin-sensitized mice after challenge showed bronchial hyperreactivity and increased IL-5 in the serum BALF, as well as eosinophil infiltration in the lungs. However, nasal histology of the ovalbumin-sensitized mice showed no increase in eosinophil infiltration. The nasal lavage fluid revealed no increase in eosinophils or IL-5, and the nasal airway resistance did not increase after challenge either. In a mouse allergy model, exclusive allergen challenge of the lower airway can elicit a pulmonary and systemic allergic response, but does not induce upper airway inflammatory or physiological responses.
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影响因子：
14.2
作者：
Braunstahl, GJ;Overbeek, SE;Fokkens, WJ
通讯作者：
Fokkens, WJ
DOI：
10.1164/ajrccm.161.6.9906121
发表时间：
2000-06-01
影响因子：
24.7
作者：
Braunstahl, GJ;Kleinjan, A;Fokkens, WJ
通讯作者：
Fokkens, WJ
无限制单腔体积描记法是否可以有效评估过敏性 BALB/c 小鼠的气道反应性？
影响因子：
5.8
作者：
Zhang Q;Lai K;Xie J;Chen G;Zhong N
通讯作者：
Zhong N
影响因子：
6.1
作者：
Hellings, PW;Hessel, EM;Ceuppens, JL
通讯作者：
Ceuppens, JL
DOI：
10.2500/108854194778816634
发表时间：
1994-01-01
期刊：
ALLERGY PROCEEDINGS
影响因子：
--
作者：
SETTIPANE, RJ;HAGY, GW;SETTIPANE, GA
通讯作者：
SETTIPANE, GA