Stomach wall structure and vessels imaging by acoustic resolution photoacoustic microscopy.
Stomach wall structure and vessels imaging by acoustic resolution photoacoustic microscopy.
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DOI：
10.3748/wjg.v24.i31.3531
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发表时间：
2018-08-21
影响因子：
4.3
通讯作者：
Zheng G
中科院分区：
文献类型：
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作者：
Wang C;Lu YF;Cai CM;Xiang HZ;Zheng G
逐层成像胃壁血管和组织。我们建立了声学分辨率光声显微镜（AR-PAM）系统，用于成像层状组织，如胃壁。将可调谐染料激光系统耦合到光纤束上。束的纤维被放置在9个方向，以45°的入射角围绕一个高频超声换能器连接到声学透镜。这种结构在声透镜下的组织表面形成一个暗场，来自纤维的9束光在声透镜焦点附近组合。样品片从猪胃的一部分切到培养皿中。为了实现肿瘤的光声深度成像，我们设计了一种基于吲哚菁绿（ICG）染料的肿瘤模型。将浓度为129 μM/mL的ICG溶液混合成熔融凝胶，然后将浓度为12.9 μM/mL的ICG凝胶混合物注入胃粘膜下层。注射量控制在0.1 mL，制作小肿瘤模型。建立了一种基于光纤照明的声分辨率光声显微镜，在500 μm的焦区范围内，其轴向分辨率为25 μm，横向分辨率为50 μm。我们把激光波长调到600纳米。驱动光声探针在200 μm的组织厚度上进行b扫描成像。获得组织粘膜和粘膜下层的光声微像，并与苏木精-伊红染色组织的病理照片进行比较。我们已经观察到更详细的组织内部结构。我们也利用这种光声显微镜成像粘膜下层的血管。粘膜下肿瘤模型采用ICG染色高对比成像。这种AR-PAM无需任何造影剂即可对胃壁粘膜下的一些血管和结构进行逐层成像。
To image stomach wall blood vessels and tissue, layer-by-layer. We built up the acoustic resolution photoacoustic microscopy (AR-PAM) system for imaging layered tissues, such as the stomach wall. A tunable dye laser system was coupled to a fiber bundle. The fibers of the bundle were placed in nine directions with an incident angle of 45° around a high-frequency ultrasound transducer attached to the acoustic lens. This structure formed a dark field on the tissue surface under the acoustic lens and the nine light beams from the fibers to be combined near the focal point of the acoustic lens. The sample piece was cut from a part of the porcine stomach into a petri dish. In order to realize photoacoustic depth imaging of tumor, we designed a tumor model based on indocyanine green (ICG) dye. The ICG solution (concentration of 129 μM/mL) was mixed into molten gel, and then a gel mixture of ICG (concentration of 12.9 μM/mL) was injected into the stomach submucosa. The injection quantity was controlled by 0.1 mL to make a small tumor model. An acoustic resolution photoacoustic microscopy based on fiber illumination was established and an axial resolution of 25 μm and a lateral resolution of 50 μm in its focal zone range of 500 μm has been accomplished. We tuned the laser wavelength to 600 nm. The photoacoustic probe was driven to do B-scan imaging in tissue thickness of 200 μm. The photoacoustic micro-image of mucosa and submucosa of the tissue have been obtained and compared with a pathological photograph of the tissue stained by hematoxylin-eosin staining. We have observed more detailed internal structure of the tissue. We also utilized this photoacoustic microscopy to image blood vessels inside the submucosa. High contrast imaging of the submucosa tumor model was obtained using ICG dye. This AR-PAM is able to image layer-by-layer construction and some blood vessels under mucosa in the stomach wall without any contrast agents.
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影响因子：
3.6
作者：
Xing W;Wang L;Maslov K;Wang LV
通讯作者：
Wang LV
影响因子：
3.6
作者：
Wang Y;Hu S;Maslov K;Zhang Y;Xia Y;Wang LV
通讯作者：
Wang LV
影响因子：
3.5
作者：
Wang, Xueding;Xie, Xueyi;Wang, Lihong V.
通讯作者：
Wang, Lihong V.
影响因子：
3.6
作者：
Maslov, Konstantin;Zhang, Hao F.;Wang, Lihong V.
通讯作者：
Wang, Lihong V.
影响因子：
82.9
作者：
通讯作者：
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