Biphasic regulation of the transcription factor ABORTED MICROSPORES (AMS) is essential for tapetum and pollen development in Arabidopsis.
Biphasic regulation of the transcription factor ABORTED MICROSPORES (AMS) is essential for tapetum and pollen development in Arabidopsis.
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DOI：
10.1111/nph.14200
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发表时间：
2017-01
期刊：
影响因子：
--
通讯作者：
Wilson ZA
中科院分区：
文献类型：
--
作者：
Ferguson AC;Pearce S;Band LR;Yang C;Ferjentsikova I;King J;Yuan Z;Zhang D;Wilson ZA
花粉对于植物繁殖和作物产量至关重要。它的产生需要在花药发育的特定阶段协调表达，包括早期减数分裂相关事件和晚期花粉壁形成。ABORTED MICROSPORES（AMS）转录因子是拟南芥花粉素合成、分泌和花粉壁形成的主要调控因子。在这里，我们表明，它具有复杂的调节和额外的重要作用，在花粉形成的早期。创建了一种诱导型AMS报告基因，用于功能拯救、蛋白表达模式分析以及区分直接和间接靶标。数学建模用于创建基于野生型RNA和蛋白质表达的调控网络。AMS的双重活性被定义为在花药绒毡层细胞中的双相蛋白表达，在花粉减数分裂前后和花粉壁发育期间的初始峰值。直接AMS调节的靶点表现出时间调节，表明其他因素与其调节相关。我们表明，AMS双相表达是必不可少的花粉发育，并定义不同的功能活动在早期和晚期花粉发育。数学模型表明，AMS可能与其他绒毡层表达的转录因子竞争性地形成蛋白质复合物，并且双相调节是由于上游调节因子的抑制和AMS蛋白质降解的促进。
Viable pollen is essential for plant reproduction and crop yield. Its production requires coordinated expression at specific stages during anther development, involving early meiosis‐associated events and late pollen wall formation. The ABORTED MICROSPORES (AMS) transcription factor is a master regulator of sporopollenin biosynthesis, secretion and pollen wall formation in Arabidopsis. Here we show that it has complex regulation and additional essential roles earlier in pollen formation. An inducible‐AMS reporter was created for functional rescue, protein expression pattern analysis, and to distinguish between direct and indirect targets. Mathematical modelling was used to create regulatory networks based on wild‐type RNA and protein expression. Dual activity of AMS was defined by biphasic protein expression in anther tapetal cells, with an initial peak around pollen meiosis and then later during pollen wall development. Direct AMS‐regulated targets exhibit temporal regulation, indicating that additional factors are associated with their regulation. We demonstrate that AMS biphasic expression is essential for pollen development, and defines distinct functional activities during early and late pollen development. Mathematical modelling suggests that AMS may competitively form a protein complex with other tapetum‐expressed transcription factors, and that biphasic regulation is due to repression of upstream regulators and promotion of AMS protein degradation.
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影响因子：
7.2
作者：
Wilson, ZA;Morroll, SM;Tighe, PJ
通讯作者：
Tighe, PJ
影响因子：
7.4
作者：
Alves-Ferreira, Marcio;Wellmer, Frank;Meyerowitz, Elliot M.
通讯作者：
Meyerowitz, Elliot M.
影响因子：
7.2
作者：
Feng, Baomin;Lu, Dihong;Ma, Hong
通讯作者：
Ma, Hong
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
1.6
作者：
TARNOWSKI, BI;SPINALE, FG;NICHOLSON, JH
通讯作者：
NICHOLSON, JH