Evidence for existence of a close association between CD14 and CXCR4 on monocytic cell line U937
Evidence for existence of a close association between CD14 and CXCR4 on monocytic cell line U937
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单核细胞系 U937 上 CD14 和 CXCR4 之间存在密切关联的证据
DOI：
10.1080/10002007088537500
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发表时间：
2007-08
期刊：
影响因子：
--
通讯作者：
Yang Hengwen
中科院分区：
文献类型：
--
作者：
Lan Canhui;Chen Yinghua;Yang Hengwen
HIV-1辅助受体CXCR 4和CCR 5在单核细胞表面的表达受CD 14配体的调节，从而改变细胞对HIV-1的易感性。我们前期的研究发现，抗CD 14单克隆抗体能显著抑制CXCR 4介导的趋化性和细胞融合。基于这些研究，我们探索了单核细胞系上CD 14和CXCR 4之间的潜在关系，FITC偶联的抗CD 14单克隆抗体（mAb）12 G5强烈抑制了FITC偶联的抗CD 14单克隆抗体（TUK 4和UCHM 1）与U937的结合，而另一种CX-CR 4特异性mAb B-R24对这种结合没有表现出任何影响。另一方面，两种抗CD 14单克隆抗体（TK 4和UCH-M1）明显抑制PE缀合的抗CXCR 4 mAb 12 G5与U937的结合，但不抑制mAb 12 G5与CXCR 4转染的3 T3细胞（3 T3. T4，CXCR 4），这表明CD 14特异性mAb对mAb 12 G5与CXCR 4结合的阻断不涉及......
Abstract The surface expression of HIV-1 coreceptors (CXCR4 and CCR5) on monocytes can be regulated by the ligand of CD14, and the susceptibility of the cells to HIV-1 is then changed. Our previous study found that monoclonal antibody against CD14 could dramatically inhibit CXCR4-medicated chemotaxis and cell-cell fusion. Based on these studies, we explored potential relationship between CD14 and CXCR4 on monocytic cell lineFITC-conjugated anti-CD14 monocolnal antibody (mAb) 12G5 strongly inhibited binding of the FITC-conjugated anti-CD14 monoclonal antibodies (TUK4 and UCHM1) to U937, while another CX-CR4-specific mAb B-R24 did not show any effect on this binding. On the other hand, two anti-CD14 monoclonal antibodies (TK4 and UCH-M1) obviously inhibited the binding of the PE-conjugated anti-CXCR4 mAb 12G5 to U937 but did not inhibit the binding of mAb 12G5 to CXCR4-transfected 3T3 cells (3T3. T4, CXCR4), which indicates that the blocking of mAb 12G5 binding to CXCR4 by CD14-specific mAbs is not involved...
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影响因子：
15.3
作者：
Verani, A;Scarlatti, G;Comar, M;Tresoldi, E;Polo, S;Giacca, M;Lusso, P;Siccardi, AG;Vercelli, D
通讯作者：
Vercelli, D
影响因子：
56.9
作者：
WRIGHT, SD;RAMOS, RA;MATHISON, JC
通讯作者：
MATHISON, JC
影响因子：
5.8
作者：
Landmann, R;Müller, B;Zimmerli, W
通讯作者：
Zimmerli, W
影响因子：
30.5
作者：
Triantafilou, K;Triantafilou, M;Dedrick, RL
通讯作者：
Dedrick, RL
影响因子：
32.4
作者：
PUGIN, J;HEUMANN, D;ULEVITCH, RJ
通讯作者：
ULEVITCH, RJ