Diversity of the bacterial and fungal microflora from the midgut and cuticle of phlebotomine sand flies collected in North-Western Iran.
Diversity of the bacterial and fungal microflora from the midgut and cuticle of phlebotomine sand flies collected in North-Western Iran.
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DOI：
10.1371/journal.pone.0050259
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Abyaneh MR
中科院分区：
文献类型：
--
作者：
Akhoundi M;Bakhtiari R;Guillard T;Baghaei A;Tolouei R;Sereno D;Toubas D;Depaquit J;Abyaneh MR
白蛉是利什曼病的媒介，利什曼病是由利什曼原虫属引起的寄生虫病。很少有人知道的流行和多样性的沙蝇微生物区系殖民中肠或角质层。特别是，关于真菌多样性的信息很少。这一信息对于制定病媒控制战略十分重要。五种沙蝇：在伊朗西北部的Bileh Savar和Kaleybar捕获了Papatasi白蛉、P. sergenti白蛉、P. kandelakii白蛉、P. perfiliewi白蛉和P. halepensis白蛉，这些白蛉位于内脏利什曼病的地方性疫源地。共处理了35份标本。细菌和真菌菌株通过常规微生物学方法鉴定。我们的特点是39真菌分离角质层和/或中肠。分离到6个不同的属，包括青霉属（Penicillium）17株，曲霉属（Aspergillus）14株，枝顶孢属（Acremonium）5株，镰刀菌属（Fusarium）1株，地霉属（Geotrichum）1株和假丝酵母属（Candida）1株。从10株分离菌中鉴定出33株革兰氏阴性菌：粘质沙雷氏菌（9株）、阴沟肠杆菌（6株）、荧光假单胞菌（6株）、臭鼻克雷伯氏菌（4株）、不动杆菌属（3株）、大肠埃希氏菌（3株）、Asaia sp.（1株）和泛菌属（1株）;我们的研究提供了关于实地收集的白蛉的微生物多样性的新数据，并首次证明了白蛉中存在Asaia sp.。我们还发现白蛉的生理阶段（未进食、新鲜进食、半妊娠和妊娠）与它们携带的细菌数量之间存在联系。有趣的是，Pantoea sp.和Klebsiella ozaenae已经在旧大陆的沙蝇物种中分离出来。后者物种的存在下，白蛉角质层，并在女性中肠建议的作用，这种节肢动物传播这些病原菌在流行地区。需要进一步的实验来清楚地描绘白蛉的媒介作用（被动或主动）。
Phlebotomine sand flies are the vectors of the leishmaniases, parasitic diseases caused by Leishmania spp. Little is known about the prevalence and diversity of sand fly microflora colonizing the midgut or the cuticle. Particularly, there is little information on the fungal diversity. This information is important for development of vector control strategies. Five sand fly species: Phlebotomus papatasi, P. sergenti, P. kandelakii, P. perfiliewi and P. halepensis were caught in Bileh Savar and Kaleybar in North-Western Iran that are located in endemic foci of visceral leishmaniasis. A total of 35 specimens were processed. Bacterial and fungal strains were identified by routine microbiological methods. We characterized 39 fungal isolates from the cuticle and/or the midgut. They belong to six different genera including Penicillium (17 isolates), Aspergillus (14), Acremonium (5), Fusarium (1), Geotrichum (1) and Candida (1). We identified 33 Gram-negative bacteria: Serratia marcescens (9 isolates), Enterobacter cloacae (6), Pseudomonas fluorescens (6), Klebsiella ozaenae (4), Acinetobacter sp. (3), Escherichia coli (3), Asaia sp. (1) and Pantoea sp. (1) as well as Gram-positive bacteria Bacillus subtilis (5) and Micrococcus luteus (5) in 10 isolates. Our study provides new data on the microbiotic diversity of field-collected sand flies and for the first time, evidence of the presence of Asaia sp. in sand flies. We have also found a link between physiological stages (unfed, fresh fed, semi gravid and gravid) of sand flies and number of bacteria that they carry. Interestingly Pantoea sp. and Klebsiella ozaenae have been isolated in Old World sand fly species. The presence of latter species on sand fly cuticle and in the female midgut suggests a role for this arthropod in dissemination of these pathogenic bacteria in endemic areas. Further experiments are required to clearly delineate the vectorial role (passive or active) of sand flies.
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DOI：
10.4269/ajtmh.2008.79.881
发表时间：
2008-12-01
影响因子：
3.3
作者：
Hillesland, Heidi;Read, Amber;Durvasula, Ravi
通讯作者：
Durvasula, Ravi
影响因子：
1.8
作者：
Gouveia, Cheryl;Asensi, Marise D.;Oliveira, Sandra M.P. de
通讯作者：
Oliveira, Sandra M.P. de
影响因子：
2.1
作者：
BEIER, MS;PUMPUNI, CB;DAVIS, JR
通讯作者：
DAVIS, JR
DOI：
10.4269/ajtmh.1996.54.219
发表时间：
1996-02-01
影响因子：
3.3
作者：
DeMaio, J;Pumpuni, CB;Beier, JC
通讯作者：
Beier, JC
DOI：
10.1016/j.trstmh.2008.04.026
发表时间：
2008-09-01
影响因子：
2.2
作者：
Parvizi, P.;Mazloumi-Gavgani, A. S.;Ready, P. D.
通讯作者：
Ready, P. D.