ATAF1 transcription factor directly regulates abscisic acid biosynthetic gene NCED3 in Arabidopsis thaliana.
ATAF1 transcription factor directly regulates abscisic acid biosynthetic gene NCED3 in Arabidopsis thaliana.
复制标题
DOI：
10.1016/j.fob.2013.07.006
复制
发表时间：
2013
期刊：
影响因子：
2.6
通讯作者：
Skriver K
中科院分区：
文献类型：
--
作者：
Jensen MK;Lindemose S;de Masi F;Reimer JJ;Nielsen M;Perera V;Workman CT;Turck F;Grant MR;Mundy J;Petersen M;Skriver K
ATAF1是拟南芥（Arabidopsis thaliana） NAC转录因子，在植物适应环境胁迫和发育过程中发挥重要作用。为了寻找ATAF1靶基因，我们使用了蛋白结合微阵列和染色质免疫沉淀（ChIP）技术。鉴定T[A，C,G]CGT[A，G]和TT[A，C，G]CGT为ATAF1一致结合序列。通过公开的微阵列实验进行共表达分析，鉴定出25个与ATAF1共表达的基因。ATAF1共表达子的启动子区域显著富集ATAF1结合位点，TTGCGTA位于植物激素关键脱落酸（ABA）生物合成基因NCED3的启动子中。ChIP-qPCR和表达分析显示，ATAF1结合NCED3启动子与NCED3表达和ABA激素水平升高相关。这些结果表明ATAF1调控ABA的生物合成。鉴定了拟南芥转录因子ATAF1的一致dna结合位点。ATAF1共表达簇富集了ATAF1的一致结合位点ABA生物合成基因NCED3与ATAF1共表达。过表达ATAF1的植物增加了ABA激素水平。ATAF1在体内结合NCED3的启动子激活表达。
ATAF1, an Arabidopsis thaliana NAC transcription factor, plays important roles in plant adaptation to environmental stress and development. To search for ATAF1 target genes, we used protein binding microarrays and chromatin-immunoprecipitation (ChIP). This identified T[A,C,G]CGT[A,G] and TT[A,C,G]CGT as ATAF1 consensus binding sequences. Co-expression analysis across publicly available microarray experiments identified 25 genes co-expressed with ATAF1. The promoter regions of ATAF1 co-expressors were significantly enriched for ATAF1 binding sites, and TTGCGTA was identified in the promoter of the key abscisic acid (ABA) phytohormone biosynthetic gene NCED3. ChIP-qPCR and expression analysis showed that ATAF1 binding to the NCED3 promoter correlated with increased NCED3 expression and ABA hormone levels. These results indicate that ATAF1 regulates ABA biosynthesis. Consensus DNA-binding sites of the Arabidopsis transcription factor ATAF1 were identified. The ATAF1 co-expression cluster is enriched for ATAF1 consensus binding sites ABA biosynthetic gene NCED3 is co-expressed with ATAF1. Plants over-expressing ATAF1 have increased ABA hormone levels. ATAF1 binds in vivo to the promoter of NCED3 to activate expression.
登录
查看更多内容
影响因子：
14.8
作者：
Berger, Michael F.;Bulyk, Martha L.
通讯作者：
Bulyk, Martha L.
影响因子：
16
作者：
Badis, Gwenael;Chan, Esther T.;van Bakel, Harm;Pena-Castillo, Lourdes;Tillo, Desiree;Tsui, Kyle;Carlson, Clayton D.;Gossett, Andrea J.;Hasinoff, Michael J.;Warren, Christopher L.;Gebbia, Marinella;Talukder, Shaheynoor;Yang, Ally;Mnaimneh, Sanie;Terterov, Dimitri;Coburn, David;Yeo, Ai Li;Yeo, Zhen Xuan;Clarke, Neil D.;Lieb, Jason D.;Ansari, Aseem Z.;Nislow, Corey;Hughes, Timothy R.
通讯作者：
Hughes, Timothy R.
影响因子：
11.4
作者：
de Torres-Zabala, Marta;Truman, William;Grant, Murray
通讯作者：
Grant, Murray
影响因子：
3.7
作者：
Domagalska, Malgorzata A.;Sarnowska, Elzbieta;Davis, Seth J.
通讯作者：
Davis, Seth J.
影响因子：
7.2
作者：
Jossier, Mathieu;Bouly, Jean-Pierre;Thomas, Martine
通讯作者：
Thomas, Martine