Exploring the Diversity of the Human Blood Virome.
Exploring the Diversity of the Human Blood Virome.
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DOI：
10.3390/v13112322
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发表时间：
2021-11-21
期刊：
影响因子：
--
通讯作者：
Cuevas JM
中科院分区：
文献类型：
--
作者：
Cebriá-Mendoza M;Bracho MA;Arbona C;Larrea L;Díaz W;Sanjuán R;Cuevas JM
宏基因组学正在极大地提高我们发现新病毒的能力，以及它们与疾病可能存在的关联。然而，宏基因组学也改变了我们对病毒的总体认识。目前已知病毒多样性的巨大扩展揭示了很大一部分非致病性病毒，并提供了一个新的视角，即病毒作为许多生态系统的重要组成部分发挥作用。在这种情况下，对人类血液病毒组的研究往往是为了寻找新的病毒性疾病，特别是那些与输血有关的疾病。然而，这些研究表明，血液中普遍存在明显的非致病性病毒，特别是人类类线虫病毒，以及在较低程度上存在的人类佩吉病毒（HPgV）。为了阐明人类血液病毒组的多样性，我们将来自西班牙587名健康献血者的血浆样本纳入病毒富集方案，然后进行大规模平行测序。这表明，蛇状病毒显然是血液病毒组的主要组成部分，并表现出显著的多样性。我们总共组装了332个完整或接近完整的无绒病毒基因组，其中50个可以被认为是新物种。hpv的出现频率要低得多，但尽管如此，我们还是恢复了17种不同的分离株，我们随后用这些分离株来表征这种病毒的多样性。对人类血液病毒的深入研究应该有助于阐明这些病毒的生态学，并揭示潜在的相关疾病。
Metagenomics is greatly improving our ability to discover new viruses, as well as their possible associations with disease. However, metagenomics has also changed our understanding of viruses in general. The vast expansion of currently known viral diversity has revealed a large fraction of non-pathogenic viruses, and offers a new perspective in which viruses function as important components of many ecosystems. In this vein, studies of the human blood virome are often motivated by the search for new viral diseases, especially those associated with blood transfusions. However, these studies have revealed the common presence of apparently non-pathogenic viruses in blood, particularly human anelloviruses and, to a lower extent, human pegiviruses (HPgV). To shed light on the diversity of the human blood virome, we subjected pooled plasma samples from 587 healthy donors in Spain to a viral enrichment protocol, followed by massive parallel sequencing. This showed that anelloviruses were clearly the major component of the blood virome and showed remarkable diversity. In total, we assembled 332 complete or near-complete anellovirus genomes, 50 of which could be considered new species. HPgV was much less frequent, but we, nevertheless, recovered 17 different isolates that we subsequently used for characterizing the diversity of this virus. In-depth investigation of the human blood virome should help to elucidate the ecology of these viruses, and to unveil potentially associated diseases.
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影响因子：
30.3
作者：
Arze, Cesar A.;Springer, Simeon;Yozwiak, Nathan L.
通讯作者：
Yozwiak, Nathan L.
影响因子：
4.6
作者：
Cebriá-Mendoza M;Arbona C;Larrea L;Díaz W;Arnau V;Peña C;Bou JV;Sanjuán R;Cuevas JM
通讯作者：
Cuevas JM
影响因子：
11.2
作者：
Chang CM;Stapleton JT;Klinzman D;McLinden JH;Purdue MP;Katki HA;Engels EA
通讯作者：
Engels EA
影响因子：
3.8
作者：
Blackard, Jason T.;Ma, Gang;Sherman, Kenneth E.
通讯作者：
Sherman, Kenneth E.
影响因子：
6.1
作者：
Forns, X;FernandezLlama, P;Rodes, J
通讯作者：
Rodes, J