Reconsidering movement of eukaryotic mRNAs between polysomes and P bodies.
Reconsidering movement of eukaryotic mRNAs between polysomes and P bodies.
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DOI：
10.1016/j.molcel.2011.09.019
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发表时间：
2011-12-09
期刊：
影响因子：
16
通讯作者：
Gilbert, Wendy V.
中科院分区：
文献类型：
--
作者：
Arribere, Joshua A.;Doudna, Jennifer A.;Gilbert, Wendy V.
细胞在不断变化的环境中生存需要适当的基因表达调控，包括转录后调控机制。在葡萄糖缺乏的酵母中的报告基因研究表明，翻译沉默的真核生物mRNAs积累在P-小体中，并可以恢复主动翻译。我们提出的证据与以下观点相矛盾，即非翻译mRNAs的可逆存储是一种普遍而普遍的现象。首先，全基因组范围的mRNA丰度、翻译和停糖后核糖体占有率的测量表明，大多数mRNAs从细胞中被耗尽，与它们从多聚体中耗尽的情况一致。其次，在没有新转录的情况下，只有不到400个基因的翻译抑制转录子的有限亚群可以在葡萄糖重新添加时被重新激活以进行翻译。这种高度选择性的转录后调控可能是一种机制，细胞可以通过暂时保留其翻译对生长具有限速作用的转录库，将在快速变化的环境中逆转基因调控决定的能量成本降至最低。
Cell survival in changing environments requires appropriate regulation of gene expression, including post-transcriptional regulatory mechanisms. From reporter gene studies in glucose-starved yeast, it was proposed that translationally silenced eukaryotic mRNAs accumulate in P-bodies and can return to active translation. We present evidence contradicting the notion that reversible storage of non-translating mRNAs is a widespread and general phenomenon. First, genome-wide measurements of mRNA abundance, translation, and ribosome occupancy following glucose withdrawal show that most mRNAs are depleted from the cell coincident with their depletion from polysomes. Second, only a limited sub-population of translationally repressed transcripts, comprising fewer than 400 genes, can be reactivated for translation upon glucose re-addition in the absence of new transcription. This highly selective post-transcriptional regulation could be a mechanism for cells to minimize the energetic costs of reversing gene-regulatory decisions in rapidly changing environments by transiently preserving a pool of transcripts whose translation is rate-limiting for growth.
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