Oral Immunization with Lactobacillus casei Expressing the Porcine Circovirus Type 2 Cap and LTB Induces Mucosal and Systemic Antibody Responses in Mice.
Oral Immunization with Lactobacillus casei Expressing the Porcine Circovirus Type 2 Cap and LTB Induces Mucosal and Systemic Antibody Responses in Mice.
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表达猪圆环病毒 2 型帽和 LTB 的干酪乳杆菌口服免疫可诱导小鼠粘膜和全身抗体反应
DOI：
10.3390/v13071302
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发表时间：
2021-07-05
期刊：
影响因子：
--
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Li F;Wang X;Ma R;Wu W;Teng F;Cheng X;Jiang Y;Zhou H;Wang L;Tang L;Qiao X;Li Y
猪圆环病毒2型（PCV2）引起断奶仔猪多种疾病，给养猪业造成严重的经济损失。本研究调查了小鼠口服表达 PCV2 衣壳蛋白 (Cap) 与大肠杆菌不耐热毒素 B 亚基 (LTB) 融合的干酪乳杆菌 ATCC393 (L. casei 393) 后的免疫反应。使用质粒pPG611.1和pPG612.1构建重组干酪乳杆菌菌株。重组 pPG611.1-Cap-LTB (pPG-1-Cap-LTB)/L 蛋白的表达和定位。干酪 393 和 pPG612.1-Cap-LTB (pPG-2-Cap-LTB)/L。检测到干酪393。发现所有重组菌株通过小鼠口服给药均具有免疫原性，并产生针对 PCV2 的粘膜和全身免疫反应。给予pPG-2-Cap-LTB/L的小鼠中特异性抗体的效价。干酪 393 的水平高于施用 pPG-1-Cap-LTB/L 的小鼠。血清和粘膜样本中的干酪393。粘膜免疫反应不仅限于胃肠道，其他粘膜部位也产生。因此，重组干酪乳杆菌的应用有助于 PCV2 疫苗的开发。
Porcine circovirus type 2 (PCV2) causes many diseases in weaned piglets, leading to serious economic losses to the pig industry. This study investigated the immune response following oral administration of Lactobacillus casei ATCC393 (L. casei 393) expressing PCV2 capsid protein (Cap) fusion with the Escherichia coli heat-labile toxin B subunit (LTB) in mice. Recombinant L. casei strains were constructed using plasmids pPG611.1 and pPG612.1. The expression and localization of proteins from recombinant pPG611.1-Cap-LTB (pPG-1-Cap-LTB)/L. casei 393 and pPG612.1-Cap-LTB (pPG-2-Cap-LTB)/L. casei 393 were detected. All recombinant strains were found to be immunogenic by oral administration in mice and developed mucosal and systemic immune responses against PCV2. The titers of specific antibodies in mice administered pPG-2-Cap-LTB/L. casei 393 were higher than those in mice administered pPG-1-Cap-LTB/L. casei 393 in serum and the mucosal samples. The mucosal immune response was not only limited to the gastrointestinal tract but was also generated in other mucosal parts. Thus, the application of recombinant L. casei could aid in vaccine development for PCV2.
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影响因子：
4.4
作者：
Lee, YK;Ho, PS;Salminen, S
通讯作者：
Salminen, S
影响因子：
2.1
作者：
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3.3
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3.1
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影响因子：
3.1
作者：
Oñate, AA;Céspedes, S;Andrews, E
通讯作者：
Andrews, E