Human thymic epithelial cells express an endogenous lectin, galectin-1, which binds to core 2 O-glycans on thymocytes and T lymphoblastoid cells.
Human thymic epithelial cells express an endogenous lectin, galectin-1, which binds to core 2 O-glycans on thymocytes and T lymphoblastoid cells.
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DOI：
10.1084/jem.181.3.877
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发表时间：
1995-03-01
影响因子：
15.3
通讯作者：
Seilhamer, Jeffrey J.
中科院分区：
文献类型：
--
作者：
Baum, Linda G.;Pang, Mabel;Perillo, Nancy L.;Wu, Terry;Delegeane, Angelo;Uittenbogaart, Christel H.;Fukuda, Minoru;Seilhamer, Jeffrey J.
胸腺上皮细胞在选择发育中的胸腺细胞中起着至关重要的作用。胸腺细胞-上皮细胞相互作用涉及许多粘附分子，包括整合素和免疫球蛋白超家族的成员。我们发现人胸腺上皮细胞合成内源性凝集素galectin-1，它与胸腺细胞和T淋巴细胞样细胞表面的寡糖配体结合。 T 类淋巴母细胞与胸腺上皮细胞的结合受到上皮细胞上半乳糖凝集素 1 抗体的抑制，以及分别识别 T 细胞表面糖蛋白 CD43 和 CD45 上碳水化合物表位的两种抗体 T305 和 2B11 的抑制。流式细胞术分析表明，T 淋巴母细胞和胸腺细胞结合重组半乳糖凝集素-1，并且在乳糖存在的情况下，凝集素结合被完全抑制。半乳糖凝集素 1 与胸腺细胞结合的程度与细胞的成熟阶段相关，因为未成熟的胸腺细胞比成熟的胸腺细胞结合更多的半乳糖凝集素 1。半乳糖凝集素-1与未成熟胸腺细胞的优先结合可能是由于这些细胞上优选寡糖配体的调节表达所致，因为我们发现T305抗体识别的表位（CD43上的核心2O-聚糖结构）在皮质细胞上表达，但在髓质细胞上不表达。 UDP-GlcNAc:Gal beta 1,3GalNAc-R beta 1, 6GlcNAc 转移酶（核心 2 beta 1, 6 GlcNAc 转移酶或 C2GnT）的表达水平，其产生核心 2 O-聚糖结构，与皮质和髓质细胞之间的糖基化变化相关。原位杂交证明，编码 C2GnT 的 mRNA 在被膜下和皮质胸腺细胞中表达较高，而在髓质胸腺细胞中表达较低。这些结果表明galectin-1参与胸腺细胞-胸腺上皮细胞相互作用，并且这种相互作用可能受到胸腺细胞表面相关寡糖配体表达的调节。
Thymic epithelial cells play a crucial role in the selection of developing thymocytes. Thymocyte-epithelial cell interactions involve a number of adhesion molecules, including members of the integrin and immunoglobulin superfamilies. We found that human thymic epithelial cells synthesize an endogenous lectin, galectin-1, which binds to oligosaccharide ligands on the surface of thymocytes and T lymphoblastoid cells. Binding of T lymphoblastoid cells to thymic epithelial cells was inhibited by antibody to galectin-1 on the epithelial cells, and by two antibodies, T305 and 2B11, that recognize carbohydrate epitopes on the T cell surface glycoproteins CD43 and CD45, respectively. T lymphoblastoid cells and thymocytes bound recombinant galectin-1, as demonstrated by flow cytometric analysis, and lectin binding was completely inhibited in the presence of lactose. The degree of galectin-1 binding to thymocytes correlated with the maturation stage of the cells, as immature thymocytes bound more galectin-1 than did mature thymocytes. Preferential binding of galectin- 1 to immature thymocytes may result from regulated expression of preferred oligosaccharide ligands on those cells, since we found that the epitope recognized by the T305 antibody, the core 2 O-glycan structure on CD43, was expressed on cortical, but not medullary cells. The level of expression of the UDP-GlcNAc:Gal beta 1,3GalNAc-R beta 1, 6GlcNAc transferase (core 2 beta 1, 6 GlcNAc transferase, or C2GnT), which creates the core 2 O-glycan structure, correlated with the glycosylation change between cortical and medullary cells. Expression of mRNA encoding the C2GnT was high in subcapsular and cortical thymocytes and low in medullary thymocytes, as demonstrated by in situ hybridization. These results suggest that galectin-1 participates in thymocyte-thymic epithelial cell interactions, and that this interaction may be regulated by expression of relevant oligosaccharide ligands on the thymocyte cell surface.
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DOI：
10.1016/s0006-291x(85)80215-1
发表时间：
1985-01-01
影响因子：
3.1
作者：
CARDING, SR;THORPE, SJ;FEIZI, T
通讯作者：
FEIZI, T
影响因子：
3.5
作者：
DEMAIO, A;LIS, H;SHARON, N
通讯作者：
SHARON, N
影响因子：
5.4
作者：
KING, PD;BATCHELOR, AH;KATZ, DR
通讯作者：
KATZ, DR
影响因子：
4.1
作者：
LEVI, G;TEICHBERG, VI
通讯作者：
TEICHBERG, VI
影响因子：
4.4
作者：
LEVI, G;TEICHBERG, VI
通讯作者：
TEICHBERG, VI