Isolation and characterization of novel human parechovirus from clinical samples.

Isolation and characterization of novel human parechovirus from clinical samples.
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DOI:
10.3201/eid1306.060896
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发表时间:
2007-06
影响因子:
11.8
通讯作者:
Fujii M
Fujii M
中科院分区:
医学2区
文献类型:
--
作者:
Watanabe K;Oie M;Higuchi M;Nishikawa M;Fujii M

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Identification of HPeV-6 will advance HPeV diagnosis and epidemiology. Using Vero cells, we isolated a virus (NII561-2000) from a cerebrospinal fluid specimen of a 1-year-old girl with Reye syndrome. The determined amino acid sequence of the virus indicated that the isolate was a human parechovirus (HPeV), a member of Picornaviridae. Neutralization test showed that the NII561-2000 virus had distinct antigenicity to HPeV-1, HPeV-2, and HPeV-3, and that the sequence was distinct from these types as well as from HPeV-4 and HPeV-5. Thus, we propose the virus (NII561-2000) as the prototype of HPeV-6. We isolated 10 NII561-2000–related viruses, 14 HPeV-1, 16 HPeV-3, and 1 HPeV-4 of 41 HPeVs from various clinical samples collected in Niigata, Japan. Clinical symptoms of the persons infected with the NII561-2000–related viruses were infectious gastroenteritis, rash, upper respiratory tract infection, and paralysis, in addition to Reye syndrome in the 1-year-old girl.
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