Temperature affects the tripartite interactions between bacteriophage WO, Wolbachia, and cytoplasmic incompatibility.
Temperature affects the tripartite interactions between bacteriophage WO, Wolbachia, and cytoplasmic incompatibility.
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DOI：
10.1371/journal.pone.0029106
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Bordenstein SR
中科院分区：
文献类型：
--
作者：
Bordenstein SR;Bordenstein SR
沃尔巴克氏体感染是理解细胞内细菌共生的模型。虽然通常从宿主和细菌的二元角度研究共生，但越来越明显的是，额外的营养水平可以影响共生。例如，节肢动物中的沃尔巴克氏体含有一种广泛分布的温带噬菌体，称为 WO，它形成病毒粒子并在合并感染之间猖獗传播。在这里，我们测试了这样一个假设：昆虫可居住范围最边缘的温度会改变噬菌体 WO 的诱导能力，进而改变沃尔巴克氏体的密度和细胞质不相容性的外显率。我们使用模型黄蜂 Nasonia vitripennis 报告了四个关键发现：首先，与 25℃ 下饲养的黄蜂相比，18℃ 的冷处理和 30℃ 的热处理均可将沃尔巴克氏体密度降低多达 74%。其次，在所有因温度变化而导致沃尔巴克氏体密度下降的情况下，噬菌体 WO 密度都会增加，并与沃尔巴克氏体密度呈负相关。热量对噬菌体 WO 有显着影响，产生的噬菌体密度比室温对照高 552%。第三，昆虫家族对噬菌体WO和内生生物密度有显着影响。第四，在极端温度下，温度介导的密度阈值调整使沃尔巴克氏体引起完全的细胞质不相容。综上所述，这些结果表明温度同时影响噬菌体 WO 密度、内共生体密度和细胞质不相容性的外显率。虽然温度冲击增强了多种医学和工业上重要细菌的噬菌体诱导性和随之而来的细菌死亡率，但这是对专性细胞内细菌中这种关联的首次研究。讨论了 Wolbachia 中 SOS 全球传感反馈机制的含义。
Wolbachia infections are a model for understanding intracellular, bacterial symbioses. While the symbiosis is often studied from a binary perspective of host and bacteria, it is increasingly apparent that additional trophic levels can influence the symbiosis. For example, Wolbachia in arthropods harbor a widespread temperate bacteriophage, termed WO, that forms virions and rampantly transfers between coinfections. Here we test the hypothesis that temperatures at the extreme edges of an insect's habitable range alter bacteriophage WO inducibility and in turn, Wolbachia densities and the penetrance of cytoplasmic incompatibility. We report four key findings using the model wasp, Nasonia vitripennis: First, both cold treatment at 18 C and heat treatment at 30 C reduce Wolbachia densities by as much as 74% relative to wasps reared at 25 C. Second, in all cases where Wolbachia densities decline due to temperature changes, phage WO densities increase and inversely associate with Wolbachia densities. Heat has a marked effect on phage WO, yielding phage densities that are 552% higher than the room temperature control. Third, there is a significant affect of insect family on phage WO and endoysmbiont densities. Fourth, at extreme temperatures, there was a temperature-mediated adjustment to the density threshold at which Wolbachia cause complete cytoplasmic incompatibility. Taken together, these results demonstrate that temperature simultaneously affects phage WO densities, endosymbiont densities, and the penetrance of cytoplasmic incompatibility. While temperature shock enhances bacteriophage inducibility and the ensuing bacterial mortality in a wide range of medically and industrially-important bacteria, this is the first investigation of the associations in an obligate intracellular bacteria. Implications to a SOS global sensing feedback mechanism in Wolbachia are discussed.
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影响因子：
9.8
作者：
Ogata H;Renesto P;Audic S;Robert C;Blanc G;Fournier PE;Parinello H;Claverie JM;Raoult D
通讯作者：
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影响因子：
3.4
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影响因子：
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DOI：
10.1016/j.bbrc.2004.03.164
发表时间：
2004-05-14
影响因子：
3.1
作者：
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通讯作者：
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影响因子：
10.7
作者：
Gavotte, Laurent;Henri, Helene;Vavre, Fabrice
通讯作者：
Vavre, Fabrice