Evidence that a transcription factor regulatory network coordinates oxidative stress response and secondary metabolism in aspergilli.
Evidence that a transcription factor regulatory network coordinates oxidative stress response and secondary metabolism in aspergilli.
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DOI：
10.1002/mbo3.63
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发表时间：
2013-02
期刊：
影响因子：
3.4
通讯作者：
Linz, John E.
中科院分区：
文献类型：
--
作者：
Hong, Sung-Yong;Roze, Ludmila V.;Wee, Josephine;Linz, John E.
黄曲霉毒素是一种次生代谢物和强致癌物。我们研究了寄生曲霉应激反应与黄曲霉毒素合成相关基因协同激活的一种机制。电泳迁移转移实验表明，碱性亮氨酸拉链（bZIP）转录因子AtfB是一个主要的共同调节因子，它结合了早期（fas-1）、中期（ver1）和晚期（omtA）黄曲霉毒素生物合成基因的启动子，以及cAMP反应元件基元上的应激反应基因（菌丝特异性cat1和线粒体特异性Mn sod）。在黄曲霉毒素生物合成和应激反应基因启动子中发现了一个新的保守基序5′-T/GNT/CAAG CCNNG/AA/GC/ANT/C-3′。转录因子的搜索发现SrrA是一个可以结合基序的转录因子。此外，我们还在黄曲霉毒素生物合成和应激反应基因的启动子中发现了一个STRE基序（5 ' -CCCCT-3 '），竞争EMSA表明MsnA与该基序结合。我们的研究首次提供了强有力的证据，表明至少有四种转录因子（AtfB, SrrA， AP-1和MsnA）参与了一个诱导黄曲霉毒素生物合成的调控网络，作为寄生蜂对氧化应激的细胞反应的一部分。
The mycotoxin aflatoxin is a secondary metabolite and potent human carcinogen. We investigated one mechanism that links stress response with coordinate activation of genes involved in aflatoxin biosynthesis in Aspergillus parasiticus. Electrophoretic mobility shift assays demonstrated that AtfB, a basic leucine zipper (bZIP) transcription factor, is a master co-regulator that binds promoters of early (fas-1), middle (ver-1), and late (omtA) aflatoxin biosynthetic genes as well as stress-response genes (mycelia-specific cat1 and mitochondria-specific Mn sod) at cAMP response element motifs. A novel conserved motif 5′-T/GNT/CAAG CCNNG/AA/GC/ANT/C-3′ was identified in promoters of the aflatoxin biosynthetic and stress-response genes. A search for transcription factors identified SrrA as a transcription factor that could bind to the motif. Moreover, we also identified a STRE motif (5′-CCCCT-3′) in promoters of aflatoxin biosynthetic and stress-response genes, and competition EMSA suggested that MsnA binds to this motif. Our study for the first time provides strong evidence to suggest that at least four transcription factors (AtfB, SrrA, AP-1, and MsnA) participate in a regulatory network that induces aflatoxin biosynthesis as part of the cellular response to oxidative stress in A. parasiticus.
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影响因子：
3.6
作者：
Lara-Rojas F;Sánchez O;Kawasaki L;Aguirre J
通讯作者：
Aguirre J
DOI：
10.1073/pnas.88.9.3720
发表时间：
1991-05-01
影响因子：
11.1
作者：
HAI, T;CURRAN, T
通讯作者：
CURRAN, T
影响因子：
3.6
作者：
He, XJ;Fassler, JS
通讯作者：
Fassler, JS
影响因子：
7.4
作者：
Jayashree, T;Subramanyam, C
通讯作者：
Subramanyam, C
影响因子：
1.6
作者：
Hagiwara, Daisuke;Matsubayashi, Yoshihiro;Mizuno, Takeshi
通讯作者：
Mizuno, Takeshi