Evaluation of Novel Antibacterial N-Halamine Nanoparticles Prodrugs towards Susceptibility of Escherichia coli Induced by DksA Protein.
Evaluation of Novel Antibacterial N-Halamine Nanoparticles Prodrugs towards Susceptibility of Escherichia coli Induced by DksA Protein.
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新型抗菌N-卤胺纳米粒前药对DksA蛋白诱导大肠杆菌敏感性的评价
DOI：
10.3390/molecules20047292
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发表时间：
2015-04-21
期刊：
影响因子：
--
通讯作者：
Morigen
中科院分区：
文献类型：
--
作者：
Dong Q;Dong A;Morigen
通过将 N-卤胺固定在聚苯乙烯涂层的二氧化硅纳米颗粒（SiO2@PS NP）上，成功合成了可能用于杀死病原菌的新型 N-卤胺纳米颗粒，即 SiO2@PS/N-卤胺 NP。系统研究了氯化温度、漂白浓度、氯化时间等反应条件对产物中氧化性氯含量的影响。以大肠杆菌（E. coli）为模型菌，通过改良平板计数法测试产品的抗菌活性。还使用扫描电子显微镜结合抑菌圈研究研究了产品抗菌作用的可能机制。通过调节产品中的氧化氯含量可以很好地控制产品的抗菌能力。更重要的是，DksA 蛋白在大肠杆菌对产品的敏感性中的作用已通过时间灭活测定得到证实。这项对大肠杆菌对 N-卤胺 NP 敏感性的深入研究提供了对 N-卤胺在灭活细菌甚至疾病控制方面的效用的系统了解。
Novel N-halamine nanoparticles potentially useful for killing pathogenic bacteria, i.e., SiO2@PS/N-halamine NPs, were successfully synthesized via the immobilization of N-halamines onto the polystyrene-coated silica nanoparticles (SiO2@PS NPs). The effect of reaction conditions, i.e., chlorination temperature, bleaching concentration, chlorination time, on the oxidative chlorine content in the products was systematically investigated. The antibacterial activity of the products was tested via the modified plate counting methd using Escherichia coli (E. coli) as a model bacterium. The possible mechanism of the antibacterial action of the products was also studied using scanning electron microscopy combined with a inhibition zone study. The antimicrobial capability of the products was well controlled by tuning the oxidative chlorine content in the products. More importantly, the role of DksA protein in the susceptibility of E. coli against the products was proven using a time-kill assay. This in-depth investigation of the sensitivity of E. coli towards N-halamine NPs provides a systematic understanding of the utility of N-halamines for deactivating bacteria or even disease control.
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