Optimization of micropatterned poly(lactic-co-glycolic acid) films for enhancing dorsal root ganglion cell orientation and extension.
Optimization of micropatterned poly(lactic-co-glycolic acid) films for enhancing dorsal root ganglion cell orientation and extension.
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DOI：
10.4103/1673-5374.224377
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发表时间：
2018-01
影响因子：
6.1
通讯作者：
Agarwal J
中科院分区：
文献类型：
--
作者：
Li CW;Davis B;Shea J;Sant H;Gale BK;Agarwal J
神经导管是一种可行的替代“金标准”自体移植物治疗小周围神经间隙损伤。然而，它们往往产生不充分的功能恢复结果，并且在大间隙损伤中无效。脊/槽表面微图案化已被证明可以促进神经细胞定向和引导生长。然而，在聚乳酸-羟基乙酸（PLGA）薄膜上，通过优化脊/槽参数的比例来促进DRG细胞的定向和伸展，这方面的研究尚无先例。采用光刻技术和微模塑技术确定了PLGA薄膜上脊/槽尺寸的各种组合。将鸡胚DRG细胞培养在微图型PLGA膜上，进行细胞定向和迁移评价。在测试期间，薄膜发生了生物降解，然而，这并没有引起微图案的变形或扭曲。DRG电池取向测试结果表明，当脊槽比等于1（脊槽宽度参数相等，即10 μm/10 μm（偶数））时，取向程度取决于脊槽尺寸，当该比值小于1（槽控制）时，取向程度随脊槽尺寸减小而增大，当该比值大于1（脊控制）时，取向程度随槽宽度减小而减小。DRG神经元在10 μm/10 μm和30 μm/30 μm PLGA微图纹膜上的迁移速率和神经突延伸最大。结果表明，10 μm/10 μm和30 μm/30 μm的PLGA微图案化膜是构建神经修复装置的最佳脊/槽表面图案。
Nerve conduits have been a viable alternative to the ‘gold standard’ autograft for treating small peripheral nerve gap injuries. However, they often produce inadequate functional recovery outcomes and are ineffective in large gap injuries. Ridge/groove surface micropatterning has been shown to promote neural cell orientation and guide growth. However, optimization of the ratio of ridge/groove parameters to promote orientation and extension for dorsal root ganglion (DRG) cells on poly(lactic-co-glycolic acid) (PLGA) films has not been previously conducted. Photolithography and micro-molding were used to define various combinations of ridge/groove dimensions on PLGA films. The DRG cells obtained from chicken embryos were cultured on micropatterned PLGA films for cell orientation and migration evaluation. Biodegradation of the films occurred during the test period, however, this did not cause deformation or distortion of the micropatterns. Results from the DRG cell orientation test suggest that when the ridge/groove ratio equals 1 (ridge/groove width parameters are equal, i.e., 10 μm/10 μm (even)), the degree of alignment depends on the size of the ridges and grooves, when the ratio is smaller than 1 (groove controlled) the alignment increases as the ridge size decreases, and when the ratio is larger than 1 (ridge controlled), the alignment is reduced as the width of the grooves decreases. The migration rate and neurite extension of DRG neurons were greatest on 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films. Based on the data, the 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films are the optimized ridge/groove surface patterns for the construction of nerve repair devices.
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影响因子：
5
作者：
Makadia HK;Siegel SJ
通讯作者：
Siegel SJ
影响因子：
4.6
作者：
Joo S;Yeon Kim J;Lee E;Hong N;Sun W;Nam Y
通讯作者：
Nam Y
DOI：
10.1007/s10856-013-4922-5
发表时间：
2013-07-01
影响因子：
3.7
作者：
Mobasseri, S. A.;Terenghi, G.;Downes, S.
通讯作者：
Downes, S.
影响因子：
9.7
作者：
Shirazi, Reyhaneh Neghabat;Aldabbagh, Fawaz;McHugh, Peter
通讯作者：
McHugh, Peter
DOI：
10.2147/mder.s59124
发表时间：
2014
期刊：
Medical devices (Auckland, N.Z.)
影响因子：
--
作者：
Arslantunali D;Dursun T;Yucel D;Hasirci N;Hasirci V
通讯作者：
Hasirci V