Multiplexed Instrument-Free Bar-Chart SpinChip Integrated with Nanoparticle-Mediated Magnetic Aptasensors for Visual Quantitative Detection of Multiple Pathogens.
Multiplexed Instrument-Free Bar-Chart SpinChip Integrated with Nanoparticle-Mediated Magnetic Aptasensors for Visual Quantitative Detection of Multiple Pathogens.
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DOI：
10.1021/acs.analchem.8b02055
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发表时间：
2018-08-21
影响因子：
7.4
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Wei X;Zhou W;Sanjay ST;Zhang J;Jin Q;Xu F;Dominguez DC;Li X
开发了一种集成纳米颗粒介导的磁性适体传感器的便携式多路复用条形图SpinChip（MB-SpinChip），用于多种病原体的可视化定量无仪器检测。这种多功能的多路复用SpinChip结合了适体特异性识别和纳米颗粒催化的压力放大，以实现灵敏的即时检测（POCT）的样本到答案输出。这是第一份使用体积条形图芯片进行病原体检测的报告，也是第一个使用“旋转”机制实现多重条形图检测的条形图芯片。此外，旋转单元的引入不仅能够在多重检测中方便地将样品从一个入口引入到多个单独的通道，而且还巧妙地解决了多重体积条形图芯片中的压力交叉干扰问题。这种用户友好的MB-SpinChip可以同时对多种病原体进行高灵敏度的可视化定量检测，而无需使用任何专门的仪器。使用该MB-SpinChip，三种主要的食源性病原体包括沙门氏菌、大肠杆菌和单核细胞增生李斯特菌在苹果汁中进行了特异性定量，检测限约为10 CFU/mL。这种MB-SpinChip具有基于条形图的视觉定量读数，具有在护理点快速同时检测各种病原体的巨大潜力，并广泛应用于食品安全，环境监测和传染病诊断。
A portable multiplexed bar-chart SpinChip (MB-SpinChip) integrated with nanoparticle-mediated magnetic aptasensors was developed for visual quantitative instrument-free detection of multiple pathogens. This versatile multiplexed SpinChip combines aptamer-specific recognition and nanoparticle-catalyzed pressure amplification to achieve a sample-to-answer output for sensitive point-of-care testing (POCT). This is the first report of pathogen detection using a volumetric bar-chart chip, and it is also the first bar-chart chip using a “spinning” mechanism to achieve multiplexed bar-chart detection. Additionally, the introduction of the spin unit not only enabled convenient sample introduction from one inlet to multiple separate channels in the multiplexed detection, but also elegantly solved the pressure cross-interference problem in the multiplexed volumetric bar-chart chip. This user-friendly MB-SpinChip allows visual quantitative detection of multiple pathogens simultaneously with high sensitivity but without utilizing any specialized instruments. Using this MB-SpinChip, three major foodborne pathogens including Salmonella enterica, Escherichia coli, and Listeria monocytogenes were specifically quantified in apple juice with limits of detection of about 10 CFU/mL. This MB-SpinChip with a bar-chart-based visual quantitative readout has great potential for the rapid simultaneous detection of various pathogens at the point of care and wide applications in food safety, environmental surveillance, and infectious disease diagnosis.
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影响因子：
15
作者：
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通讯作者：
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影响因子：
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通讯作者：
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