Elasticity-based boosting of neuroepithelial nucleokinesis via indirect energy transfer from mother to daughter.
Elasticity-based boosting of neuroepithelial nucleokinesis via indirect energy transfer from mother to daughter.
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DOI：
10.1371/journal.pbio.2004426
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发表时间：
2018-04
期刊：
影响因子：
9.8
通讯作者：
Miyata T
中科院分区：
文献类型：
--
作者：
Shinoda T;Nagasaka A;Inoue Y;Higuchi R;Minami Y;Kato K;Suzuki M;Kondo T;Kawaue T;Saito K;Ueno N;Fukazawa Y;Nagayama M;Miura T;Adachi T;Miyata T
神经祖细胞（NPC），这是apicobasally延长和密集包装在发育中的大脑，系统地移动其细胞核/胞体的细胞周期依赖性的方式，称为核间迁移（IKNM）：顶端在G2和基底在G1。虽然个别IKNM的细胞内分子机制已被探索，如何异质IKNM集体协调是未知的。我们的定量细胞生物学和计算机分析显示，组织弹性机械地协助向基底IKNM的初始步骤。当M期祖细胞的索马体在根尖下空间内使用肌动球蛋白进行圆整时，由于根尖表面的收缩性，距离表面10 μm内的微区被压缩和弹性，侧向推动非M期细胞的密集相邻突起。挤压的突起然后向中心和基部弹回以推动祖细胞的子细胞的核/胞体。因此，从母亲到女儿的间接邻居协助的机械能转移有助于有效的大脑发育。大型脑结构的发育，如哺乳动物大脑皮层，取决于神经祖细胞持续有效的细胞生产。神经祖细胞被拉长并跨越发育中的脑壁。这些细胞的细胞核和细胞体在顶面和底面之间循环移动，并且它们每次到达顶面时都分裂。虽然我们了解单个细胞如何实现这个周期，但几个祖细胞的运动如何相互协调仍然是难以捉摸的。通过结合使用现场成像和机械实验，再加上数学模拟，我们表明，细胞拥挤在顶端表面，祖细胞分裂，创建一个subapical微区，是压缩和弹性。然后，我们表明，当每个母细胞聚集起来，准备分裂时，它会横向推动这个弹性微区，从而储存机械能。在细胞分裂之后，该机械能被传递到子细胞，以节能的方式沿着运动轴在基底表面的方向上沿着推动子细胞。我们的数学模拟表明，新产生的子细胞的及时离开对大脑增殖区的整体组织结构至关重要。
Neural progenitor cells (NPCs), which are apicobasally elongated and densely packed in the developing brain, systematically move their nuclei/somata in a cell cycle–dependent manner, called interkinetic nuclear migration (IKNM): apical during G2 and basal during G1. Although intracellular molecular mechanisms of individual IKNM have been explored, how heterogeneous IKNMs are collectively coordinated is unknown. Our quantitative cell-biological and in silico analyses revealed that tissue elasticity mechanically assists an initial step of basalward IKNM. When the soma of an M-phase progenitor cell rounds up using actomyosin within the subapical space, a microzone within 10 μm from the surface, which is compressed and elastic because of the apical surface’s contractility, laterally pushes the densely neighboring processes of non–M-phase cells. The pressed processes then recoil centripetally and basally to propel the nuclei/somata of the progenitor’s daughter cells. Thus, indirect neighbor-assisted transfer of mechanical energy from mother to daughter helps efficient brain development. The development of large brain structures, such as the mammalian cerebral cortex, depends on the continuous and efficient production of cells by neural progenitor cells. Neural progenitor cells are elongated and span the developing brain wall. The nuclei and bodies of these cells move cyclically between the apical and basal surfaces, and they divide every time they reach the apical surface. While we understand how individual cells achieve this cycle, how the movements of several progenitor cells are coordinated with one another remains elusive. By using a combination of live imaging and mechanical experiments, coupled with mathematical simulations, we show that cell crowding at the apical surface, where progenitor cells divide, creates a subapical microzone that is compressed and elastic. We then show that when each mother cell rounds up, preparing for division, it pushes this elastic microzone laterally, thereby storing mechanical energy. After cell division, this mechanical energy is transferred to the daughter cells, propelling them along the axis of movement in the direction of the basal surface, in an energy-saving manner. Our mathematical simulations show that timely departure of newly generated daughter cells is critical for the overall tissue structure of the cerebral proliferative zone.
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DOI：
10.1083/jcb.201509020
发表时间：
2016-02-29
期刊：
The Journal of cell biology
影响因子：
--
作者：
Katsunuma S;Honda H;Shinoda T;Ishimoto Y;Miyata T;Kiyonari H;Abe T;Nibu K;Takai Y;Togashi H
通讯作者：
Togashi H
影响因子：
--
作者：
CAVAGNA, GA;HEGLUND, NC;TAYLOR, CR
通讯作者：
TAYLOR, CR
DOI：
10.1111/dgd.12131
发表时间：
2014-05
期刊：
Development, growth & differentiation
影响因子：
--
作者：
Kawaue T;Sagou K;Kiyonari H;Ota K;Okamoto M;Shinoda T;Kawaguchi A;Miyata T
通讯作者：
Miyata T
影响因子：
64.5
作者：
CHENN, A;MCCONNELL, SK
通讯作者：
MCCONNELL, SK
影响因子：
64.5
作者：
Lui JH;Hansen DV;Kriegstein AR
通讯作者：
Kriegstein AR