Detection of human brain cancer infiltration ex vivo and in vivo using quantitative optical coherence tomography.
Detection of human brain cancer infiltration ex vivo and in vivo using quantitative optical coherence tomography.
复制标题
使用定量的光学相干断层扫描检测人脑癌的渗透和体内。
DOI：
10.1126/scitranslmed.3010611
复制
发表时间：
2015-06-17
影响因子：
17.1
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Kut C;Chaichana KL;Xi J;Raza SM;Ye X;McVeigh ER;Rodriguez FJ;Quiñones-Hinojosa A;Li X
更彻底的脑癌切除可以延长生存期，延缓复发。然而，在术中区分癌和非癌组织是具有挑战性的，尤其是在移行和浸润区。这在能言善辩的领域(例如，演讲和运动领域)尤为重要。这项研究测试了无标记定量光学相干断层扫描(OCT)用于区分人脑组织中的癌和非癌的可行性。新鲜的体外人脑组织取自32例II~IV级脑肿瘤患者和5例非肿瘤脑病患者。根据体积OCT成像数据，病理证实的脑癌组织(无论是高级别还是低级别)在肿瘤核心区和浸润区的光衰减值均显著低于非癌脑白质，OCT对脑癌患者的衰减阈值为5.5 mm-1，具有较高的敏感性和特异性。我们还使用这个衰减阈值来确认术中使用携带人脑癌的小鼠模型进行OCT引导活体手术的可行性。我们的OCT系统能够以每秒110-215帧的速率实时处理和显示彩色编码的光学特性图，或者对于8-16mm3的组织体积，能够以1.2-2.4秒的速率实时处理和显示，从而为癌症和非癌症区域提供直接的视觉线索。我们的研究证明了OCT在区分癌症和非癌症组织方面的翻译和实用潜力。与目前的临床标准相比，术中应用它可促进浸润性脑癌的安全和广泛切除，从而改善预后。
More complete brain cancer resection can prolong survival and delay recurrence. However, it is challenging to distinguish cancer from non-cancer tissues intraoperatively, especially at the transitional, infiltrative zones. This is especially critical in eloquent regions (e.g. speech and motor areas). This study tested the feasibility of label-free, quantitative optical coherence tomography (OCT) for differentiating cancer from non-cancer in human brain tissues. Fresh ex vivo human brain tissues were obtained from 32 patients with grades II-IV brain cancer and 5 patients with non-cancer brain pathologies. Based on volumetric OCT imaging data, pathologically confirmed brain cancer tissues (both high-grade and low-grade) had significantly lower optical attenuation values at both cancer core and infiltrated zones when compared with non-cancer white matter, and OCT achieved high sensitivity and specificity at an attenuation threshold of 5.5 mm-1 for brain cancer patients. We also used this attenuation threshold to confirm the intraoperative feasibility of performing in vivo OCT-guided surgery using a murine model harboring human brain cancer. Our OCT system was capable of processing and displaying a color-coded optical property map in real time at a rate of 110-215 frames per second, or 1.2-2.4 seconds for an 8-16 mm3 tissue volume, thus providing direct visual cues for cancer versus non-cancer areas. Our study demonstrates the translational and practical potential of OCT in differentiating cancer from non-cancer tissue. Its intraoperative use may facilitate safe and extensive resection of infiltrative brain cancers and consequently lead to improved outcomes when compared with current clinical standards.
登录
查看更多内容
影响因子：
3
作者：
Chaichana KL;Guerrero-Cazares H;Capilla-Gonzalez V;Zamora-Berridi G;Achanta P;Gonzalez-Perez O;Jallo GI;Garcia-Verdugo JM;Quiñones-Hinojosa A
通讯作者：
Quiñones-Hinojosa A
DOI：
10.1007/s00417-014-2640-4
发表时间：
2014-06-01
影响因子：
2.7
作者：
Reznicek, Lukas;Klein, Thomas;Neubauer, Aljoscha S.
通讯作者：
Neubauer, Aljoscha S.
DOI：
10.1002/rcs.1425
发表时间：
2012-09-01
影响因子：
2.5
作者：
Finke, M.;Kantelhardt, S.;Schweikard, A.
通讯作者：
Schweikard, A.
影响因子：
2.4
作者：
Boehringer, H. J.;Lankenau, E.;Stellmacher, F.;Reusche, E.;Huettmann, G.;Giese, A.
通讯作者：
Giese, A.
影响因子：
2.4
作者：
Boehringer, H. J.;Boller, D.;Giese, A.
通讯作者：
Giese, A.